--- _id: '2024' abstract: - lang: eng text: 'The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.' article_number: '3498' author: - first_name: Junko full_name: Toshima, Junko last_name: Toshima - first_name: Show full_name: Nishinoaki, Show last_name: Nishinoaki - first_name: Yoshifumi full_name: Sato, Yoshifumi last_name: Sato - first_name: Wataru full_name: Yamamoto, Wataru last_name: Yamamoto - first_name: Daiki full_name: Furukawa, Daiki last_name: Furukawa - first_name: Daria E full_name: Siekhaus, Daria E id: 3D224B9E-F248-11E8-B48F-1D18A9856A87 last_name: Siekhaus orcid: 0000-0001-8323-8353 - first_name: Akira full_name: Sawaguchi, Akira last_name: Sawaguchi - first_name: Jiro full_name: Toshima, Jiro last_name: Toshima citation: ama: Toshima J, Nishinoaki S, Sato Y, et al. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications. 2014;5. doi:10.1038/ncomms4498 apa: Toshima, J., Nishinoaki, S., Sato, Y., Yamamoto, W., Furukawa, D., Siekhaus, D. E., … Toshima, J. (2014). Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms4498 chicago: Toshima, Junko, Show Nishinoaki, Yoshifumi Sato, Wataru Yamamoto, Daiki Furukawa, Daria E Siekhaus, Akira Sawaguchi, and Jiro Toshima. “Bifurcation of the Endocytic Pathway into Rab5-Dependent and -Independent Transport to the Vacuole.” Nature Communications. Nature Publishing Group, 2014. https://doi.org/10.1038/ncomms4498. ieee: J. Toshima et al., “Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole,” Nature Communications, vol. 5. Nature Publishing Group, 2014. ista: Toshima J, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus DE, Sawaguchi A, Toshima J. 2014. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications. 5, 3498. mla: Toshima, Junko, et al. “Bifurcation of the Endocytic Pathway into Rab5-Dependent and -Independent Transport to the Vacuole.” Nature Communications, vol. 5, 3498, Nature Publishing Group, 2014, doi:10.1038/ncomms4498. short: J. Toshima, S. Nishinoaki, Y. Sato, W. Yamamoto, D. Furukawa, D.E. Siekhaus, A. Sawaguchi, J. Toshima, Nature Communications 5 (2014). date_created: 2018-12-11T11:55:16Z date_published: 2014-03-25T00:00:00Z date_updated: 2021-01-12T06:54:48Z day: '25' ddc: - '570' department: - _id: DaSi doi: 10.1038/ncomms4498 file: - access_level: open_access checksum: 614fb6579c86d1f95bdd95eeb9ab01b0 content_type: application/pdf creator: system date_created: 2018-12-12T10:11:11Z date_updated: 2020-07-14T12:45:25Z file_id: '4864' file_name: IST-2016-616-v1+1_DaSi_Bifurcation_Postprint.pdf file_size: 4803515 relation: main_file file_date_updated: 2020-07-14T12:45:25Z has_accepted_license: '1' intvolume: ' 5' language: - iso: eng month: '03' oa: 1 oa_version: Submitted Version publication: Nature Communications publication_status: published publisher: Nature Publishing Group publist_id: '5048' pubrep_id: '616' quality_controlled: '1' scopus_import: 1 status: public title: Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole type: journal_article user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87 volume: 5 year: '2014' ...