---
res:
  bibo_abstract:
  - The specific introduction of ^1H-^13C or ^1H-^15N moieties into otherwise deuterated
    proteins holds great potential for high-resolution solution and magic-angle spinning
    (MAS) NMR studies of protein structure and dynamics. Arginine residues play key
    roles for example at active sites of enzymes. Taking advantage of a chemically
    synthesized Arg with a ^13C-^1H2 group in an otherwise deuterated backbone, we
    demonstrate here the usefulness of proton-detected MAS NMR approaches to probe
    arginine dynamics. In experiments with crystalline ubiquitin and the 134 kDa tetrameric
    enzyme malate dehydrogenase we detected a wide range of motions, from sites that
    are rigid on time scales of at least tens of milliseconds to residues undergoing
    predominantly nanosecond motions. Spin-relaxation and dipolar-coupling measurements
    enabled quantitative determination of these dynamics. We observed microsecond
    dynamics of residue Arg54 in crystalline ubiquitin, whose backbone is known to
    sample different β-turn conformations on this time scale. The labeling scheme
    and experiments presented here expand the toolkit for high-resolution proton-detected
    MAS NMR.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Darja
      foaf_name: Rohden, Darja
      foaf_surname: Rohden
      foaf_workInfoHomepage: http://www.librecat.org/personId=81dc668a-19fa-11f0-bf31-d56534059ef3
  - foaf_Person:
      foaf_givenName: Federico
      foaf_name: Napoli, Federico
      foaf_surname: Napoli
      foaf_workInfoHomepage: http://www.librecat.org/personId=d42e08e7-f4fc-11eb-af0a-d71e26138f1b
    orcid: 0000-0002-9043-136X
  - foaf_Person:
      foaf_givenName: Anna
      foaf_name: Kapitonova, Anna
      foaf_surname: Kapitonova
      foaf_workInfoHomepage: http://www.librecat.org/personId=9fb2a840-89e1-11ee-a8b7-cc5c7ba62471
  - foaf_Person:
      foaf_givenName: Benjamin
      foaf_name: Tatman, Benjamin
      foaf_surname: Tatman
      foaf_workInfoHomepage: http://www.librecat.org/personId=71cda2f3-e604-11ee-a1df-da10587eda3f
  - foaf_Person:
      foaf_givenName: Roman J.
      foaf_name: Lichtenecker, Roman J.
      foaf_surname: Lichtenecker
  - foaf_Person:
      foaf_givenName: Paul
      foaf_name: Schanda, Paul
      foaf_surname: Schanda
      foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425
    orcid: 0000-0002-9350-7606
  bibo_doi: 10.1016/j.jmb.2025.169379
  bibo_issue: '23'
  bibo_volume: 437
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001618289100020
  dct_isPartOf:
  - http://id.crossref.org/issn/0022-2836
  - http://id.crossref.org/issn/1089-8638
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Arginine dynamics probed by magic-angle spinning NMR with a specific
    isotope-labeling scheme@
...