article published yes Maitane Muñoz Basagoiti author 1a8a7950-82cd-11ed-bd4f-9624c913a6070000-0003-1483-1457 Felix F Frey author a0270b37-8f1a-11ec-95c7-8e710c59a4f30000-0001-8501-6017 Billie Meadowcroft author a4725fd6-932b-11ed-81e2-c098c7f37ae10000-0003-3441-1337 Miguel Santana de Freitas Amaral author 4f2d02dd-47a9-11ec-ad10-82820ed3f501 Adam Prada author a43ed60a-dd22-11ed-9bf7-b34133792ea9 Anđela Šarić author bf63d406-f056-11eb-b41d-f263a6566d8b0000-0002-7854-2139 AnSa department IST-BRIDGE: International postdoctoral program project Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines project NOMIS Fellowship Program project The evolution of trafficking: from archaea to eukaryotes project Lipid membranes and membrane deformations are a long-standing area of research in soft matter and biophysics. Computer simulations have complemented analytical and experimental approaches as one of the pillars in the field. However, setting up and using membrane simulations can come with barriers due to the multidisciplinary effort involved and the vast choice of existing simulations models. In this review, we introduce the non-expert reader to coarse-grained membrane simulations at the mesoscale. Firstly, we give a concise overview of the modelling approaches to study fluid membranes, together with guidance to more specialized references. Secondly, we provide a conceptual guide on how to develop mesoscale membrane simulations. Lastly, we construct a hands-on tutorial on how to apply mesoscale membrane simulations, by providing a pedagogical examination of membrane tether pulling, shape and mechanics of membrane tubes, and membrane fluctuations with three different membrane models, and discussing them in terms of their scope and how resource-intensive they are. To ease the reader's venture into the field, we provide a repository with ready-to-run tutorials. https://research-explorer.ista.ac.at/download/20318/20912/2025_SoftMatter_MunozBasagoiti.pdf application/pdfno https://creativecommons.org/licenses/by-nc/4.0/ Royal Society of Chemistry2025 eng 1744-683X 1744-6848 2502.09798 00156284680000110.1039/d5sm00148j 21407736-7756 Muñoz Basagoiti M, Frey FF, Meadowcroft B, Santana de Freitas Amaral M, Prada A, Šarić A. 2025. A tutorial for mesoscale computer simulations of lipid membranes: Tether pulling, tubulation and fluctuations. Soft Matter. 21(40), 7736–7756. M. Muñoz Basagoiti, F.F. Frey, B. Meadowcroft, M. Santana de Freitas Amaral, A. Prada, A. Šarić, Soft Matter 21 (2025) 7736–7756. Muñoz Basagoiti, M., Frey, F. F., Meadowcroft, B., Santana de Freitas Amaral, M., Prada, A., &#38; Šarić, A. (2025). A tutorial for mesoscale computer simulations of lipid membranes: Tether pulling, tubulation and fluctuations. <i>Soft Matter</i>. Royal Society of Chemistry. <a href="https://doi.org/10.1039/d5sm00148j">https://doi.org/10.1039/d5sm00148j</a> Muñoz Basagoiti, Maitane, Felix F Frey, Billie Meadowcroft, Miguel Santana de Freitas Amaral, Adam Prada, and Anđela Šarić. “A Tutorial for Mesoscale Computer Simulations of Lipid Membranes: Tether Pulling, Tubulation and Fluctuations.” <i>Soft Matter</i>. Royal Society of Chemistry, 2025. <a href="https://doi.org/10.1039/d5sm00148j">https://doi.org/10.1039/d5sm00148j</a>. M. Muñoz Basagoiti, F. F. Frey, B. Meadowcroft, M. Santana de Freitas Amaral, A. Prada, and A. Šarić, “A tutorial for mesoscale computer simulations of lipid membranes: Tether pulling, tubulation and fluctuations,” <i>Soft Matter</i>, vol. 21, no. 40. Royal Society of Chemistry, pp. 7736–7756, 2025. Muñoz Basagoiti, Maitane, et al. “A Tutorial for Mesoscale Computer Simulations of Lipid Membranes: Tether Pulling, Tubulation and Fluctuations.” <i>Soft Matter</i>, vol. 21, no. 40, Royal Society of Chemistry, 2025, pp. 7736–56, doi:<a href="https://doi.org/10.1039/d5sm00148j">10.1039/d5sm00148j</a>. Muñoz Basagoiti M, Frey FF, Meadowcroft B, Santana de Freitas Amaral M, Prada A, Šarić A. A tutorial for mesoscale computer simulations of lipid membranes: Tether pulling, tubulation and fluctuations. <i>Soft Matter</i>. 2025;21(40):7736-7756. doi:<a href="https://doi.org/10.1039/d5sm00148j">10.1039/d5sm00148j</a> 203182025-09-10T05:34:36Z2025-12-30T10:16:52Z