{"PlanS_conform":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"last_name":"Tatman","full_name":"Tatman, Benjamin","id":"71cda2f3-e604-11ee-a1df-da10587eda3f","first_name":"Benjamin"},{"last_name":"Sridharan","full_name":"Sridharan, Vidhyalakshmi","first_name":"Vidhyalakshmi"},{"full_name":"Uttarkabat, Motilal","last_name":"Uttarkabat","first_name":"Motilal"},{"full_name":"Jaroniec, Christopher P.","last_name":"Jaroniec","first_name":"Christopher P."},{"last_name":"Ernst","full_name":"Ernst, Matthias","first_name":"Matthias"},{"id":"c316e53f-b965-11eb-b128-bb26acc59c00","first_name":"Petra","orcid":"0000-0001-8729-7326","last_name":"Rovo","full_name":"Rovo, Petra"},{"full_name":"Schanda, Paul","last_name":"Schanda","orcid":"0000-0002-9350-7606","first_name":"Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425"}],"date_created":"2025-09-10T05:37:19Z","publisher":"American Chemical Society","OA_type":"hybrid","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"Microsecond-to-millisecond motions are instrumental for many biomolecular functions, including enzymatic activity and ligand binding. Bloch-McConnell Relaxation Dispersion (BMRD) Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique for studying these dynamic processes. While BMRD experiments are routinely used to probe protein motions in solution, the experiment is more demanding in the solid state, where dipolar couplings complicate the spin dynamics. It is believed that high deuteration levels are required and sufficient to obtain accurate and quantitative data. Here we show that even under fast magic-angle spinning and high levels of deuteration artifactual “bumps” in 15N R1ρ BMRD profiles are common. The origin of these artifacts is identified as a second-order three-spin Mixed Rotational and Rotary Resonance (MIRROR) recoupling condition. These artifacts are found to be a significant confounding factor for the accurate quantification of microsecond protein dynamics using BMRD in the solid state. We show that the application of low-power continuous wave (CW) decoupling simultaneously with the 15N spin-lock leads to the suppression of these conditions and enables quantitative measurements of microsecond exchange in the solid state. Remarkably, the application of decoupling allows the measurement of accurate BMRD even in fully protonated proteins at 100 kHz MAS, thus extending the scope of μs dynamics measurements in MAS NMR."}],"external_id":{"isi":["001542746200001"],"pmid":["40748291"]},"has_accepted_license":"1","corr_author":"1","article_processing_charge":"Yes (via OA deal)","file":[{"access_level":"open_access","creator":"dernst","file_id":"20337","file_name":"2025_JACS_Tatman.pdf","date_created":"2025-09-10T07:53:10Z","success":1,"file_size":5235353,"date_updated":"2025-09-10T07:53:10Z","relation":"main_file","content_type":"application/pdf","checksum":"b350d56ddddefea96cebd62c277c0ff5"}],"type":"journal_article","oa_version":"Published Version","language":[{"iso":"eng"}],"oa":1,"quality_controlled":"1","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"month":"08","doi":"10.1021/jacs.5c09057","title":"Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR","scopus_import":"1","OA_place":"publisher","date_updated":"2025-09-30T14:37:22Z","publication_status":"published","day":"01","volume":147,"isi":1,"publication":"Journal of the American Chemical Society","intvolume":"       147","issue":"32","acknowledgement":"The authors thank Alexey Krushelnitsky for useful discussions. C.P.J. thanks NSF (MCB-2303862) and NIH (R35GM156238 and S10OD012303) for funding. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities.","citation":{"ieee":"B. Tatman <i>et al.</i>, “Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR,” <i>Journal of the American Chemical Society</i>, vol. 147, no. 32. American Chemical Society, pp. 29315–29326, 2025.","mla":"Tatman, Benjamin, et al. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” <i>Journal of the American Chemical Society</i>, vol. 147, no. 32, American Chemical Society, 2025, pp. 29315–26, doi:<a href=\"https://doi.org/10.1021/jacs.5c09057\">10.1021/jacs.5c09057</a>.","short":"B. Tatman, V. Sridharan, M. Uttarkabat, C.P. Jaroniec, M. Ernst, P. Rovo, P. Schanda, Journal of the American Chemical Society 147 (2025) 29315–29326.","apa":"Tatman, B., Sridharan, V., Uttarkabat, M., Jaroniec, C. P., Ernst, M., Rovo, P., &#38; Schanda, P. (2025). Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.5c09057\">https://doi.org/10.1021/jacs.5c09057</a>","chicago":"Tatman, Benjamin, Vidhyalakshmi Sridharan, Motilal Uttarkabat, Christopher P. Jaroniec, Matthias Ernst, Petra Rovo, and Paul Schanda. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2025. <a href=\"https://doi.org/10.1021/jacs.5c09057\">https://doi.org/10.1021/jacs.5c09057</a>.","ista":"Tatman B, Sridharan V, Uttarkabat M, Jaroniec CP, Ernst M, Rovo P, Schanda P. 2025. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. Journal of the American Chemical Society. 147(32), 29315–29326.","ama":"Tatman B, Sridharan V, Uttarkabat M, et al. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. <i>Journal of the American Chemical Society</i>. 2025;147(32):29315-29326. doi:<a href=\"https://doi.org/10.1021/jacs.5c09057\">10.1021/jacs.5c09057</a>"},"publication_identifier":{"eissn":["1520-5126"],"issn":["0002-7863"]},"pmid":1,"ddc":["540"],"status":"public","year":"2025","page":"29315-29326","_id":"20321","file_date_updated":"2025-09-10T07:53:10Z","article_type":"original","date_published":"2025-08-01T00:00:00Z","department":[{"_id":"PaSc"},{"_id":"NMR"}]}