---
res:
  bibo_abstract:
  - Rab GTPases organize intracellular trafficking and provide identity to organelles.
    Their spatiotemporal activation by guanine nucleotide exchange factors (GEFs)
    is tightly controlled to ensure fidelity. Our structural and functional comparison
    of the tri-longin domain RabGEFs Mon1-Ccz1 and Fuzzy-Inturned reveals the molecular
    basis for their target specificity. Both complexes rely on a conserved sequence
    motif of their substrate GTPases for the catalytic mechanism, while secondary
    interactions allow discrimination between targets. We also find that dimeric Mon1-Ccz1
    from fungi and the metazoan homologs with the additional third subunit RMC1/Bulli
    bind membranes through electrostatic interactions via distinct interfaces. Protein-lipid
    interaction studies and functional characterization in flies reveal an essential
    function of RMC1/Bulli as mediator of GEF complex membrane recruitment. In the
    case of Fuzzy-Inturned, reconstitution experiments demonstrate that the BAR (Bin-Amphiphysin-Rvs)
    domain protein CiBAR1 can support membrane recruitment of the GEF. Collectively,
    our study demonstrates the molecular basis for the adaptation of TLD-RabGEFs to
    different cellular functions.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Stephan
      foaf_name: Wilmes, Stephan
      foaf_surname: Wilmes
  - foaf_Person:
      foaf_givenName: Jesse
      foaf_name: Tönjes, Jesse
      foaf_surname: Tönjes
  - foaf_Person:
      foaf_givenName: Maik
      foaf_name: Drechsler, Maik
      foaf_surname: Drechsler
  - foaf_Person:
      foaf_givenName: Anita
      foaf_name: Ruf, Anita
      foaf_surname: Ruf
  - foaf_Person:
      foaf_givenName: Jan Hannes
      foaf_name: Schäfer, Jan Hannes
      foaf_surname: Schäfer
  - foaf_Person:
      foaf_givenName: Anna
      foaf_name: Lürick, Anna
      foaf_surname: Lürick
  - foaf_Person:
      foaf_givenName: Dovile
      foaf_name: Januliene, Dovile
      foaf_surname: Januliene
  - foaf_Person:
      foaf_givenName: Steven
      foaf_name: Apelt, Steven
      foaf_surname: Apelt
  - foaf_Person:
      foaf_givenName: Daniele
      foaf_name: Di Iorio, Daniele
      foaf_surname: Di Iorio
  - foaf_Person:
      foaf_givenName: Seraphine V.
      foaf_name: Wegner, Seraphine V.
      foaf_surname: Wegner
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Loose, Martin
      foaf_surname: Loose
      foaf_workInfoHomepage: http://www.librecat.org/personId=462D4284-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7309-9724
  - foaf_Person:
      foaf_givenName: Arne
      foaf_name: Moeller, Arne
      foaf_surname: Moeller
  - foaf_Person:
      foaf_givenName: Achim
      foaf_name: Paululat, Achim
      foaf_surname: Paululat
  - foaf_Person:
      foaf_givenName: Daniel
      foaf_name: Kümmel, Daniel
      foaf_surname: Kümmel
  bibo_doi: 10.1126/sciadv.adx2893
  bibo_issue: '35'
  bibo_volume: 11
  dct_date: 2025^xs_gYear
  dct_identifier:
  - UT:001559806100033
  dct_isPartOf:
  - http://id.crossref.org/issn/2375-2548
  dct_language: eng
  dct_publisher: AAAS@
  dct_title: Mechanistic adaptation of the metazoan RabGEFs Mon1-Ccz1 and Fuzzy-Inturned@
  fabio_hasPubmedId: '40864718'
...