@article{20530,
  abstract     = {Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segregation and division are mechanistically coupled in archaeal relatives of eukaryotes, which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we first describe the series of sequential changes in DNA organization that accompany cell division in Sulfolobus, which computational modeling shows likely aid genome segregation. Through a perturbation analysis we identify a regulatory checkpoint which ensures that the compaction of the genome into two spatially segregated nucleoids only occurs once cells have assembled a division ring—which also defines the axis of DNA segregation. Finally, we show that DNA compaction and segregation depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence leads to bridging DNA. Taken together, these data show how regulatory checkpoints like those operating in eukaryotes aid high-fidelity division in an archaeon.},
  author       = {Parham, Joe and Sorichetti, Valerio and Cezanne, Alice and Foo, Sherman and Kuo, Yin Wei and Hoogenberg, Baukje and Radoux-Mergault, Arthur and Mawdesley, Eloise and Gatward, Lydia Daniels and Boulanger, Jerome and Schulze, Ulrike and Šarić, Anđela and Baum, Buzz},
  issn         = {1091-6490},
  journal      = {Proceedings of the National Academy of Sciences},
  number       = {42},
  pages        = {e2513939122},
  publisher    = {National Academy of Sciences},
  title        = {{Temporal and spatial coordination of DNA segregation and cell division in an archaeon}},
  doi          = {10.1073/pnas.2513939122},
  volume       = {122},
  year         = {2025},
}