Temporal and spatial coordination of DNA segregation and cell division in an archaeon Parham, Joe Sorichetti, Valerio ; https://orcid.org/0000-0002-9645-6576 Cezanne, Alice Foo, Sherman Kuo, Yin Wei Hoogenberg, Baukje Radoux-Mergault, Arthur Mawdesley, Eloise Gatward, Lydia Daniels Boulanger, Jerome Schulze, Ulrike Šarić, Anđela ; https://orcid.org/0000-0002-7854-2139 Baum, Buzz ddc:570 Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segregation and division are mechanistically coupled in archaeal relatives of eukaryotes, which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we first describe the series of sequential changes in DNA organization that accompany cell division in Sulfolobus, which computational modeling shows likely aid genome segregation. Through a perturbation analysis we identify a regulatory checkpoint which ensures that the compaction of the genome into two spatially segregated nucleoids only occurs once cells have assembled a division ring—which also defines the axis of DNA segregation. Finally, we show that DNA compaction and segregation depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence leads to bridging DNA. Taken together, these data show how regulatory checkpoints like those operating in eukaryotes aid high-fidelity division in an archaeon. National Academy of Sciences 2025 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/20530 https://research-explorer.ista.ac.at/download/20530/20543 Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination of DNA segregation and cell division in an archaeon. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(42):e2513939122. doi:<a href="https://doi.org/10.1073/pnas.2513939122">10.1073/pnas.2513939122</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2513939122 info:eu-repo/semantics/altIdentifier/e-issn/1091-6490 info:eu-repo/semantics/altIdentifier/wos/001620648600001 info:eu-repo/semantics/altIdentifier/pmid/41091768 info:eu-repo/semantics/openAccess