{"author":[{"first_name":"Joe","full_name":"Parham, Joe","last_name":"Parham"},{"first_name":"Valerio","full_name":"Sorichetti, Valerio","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b","orcid":"0000-0002-9645-6576","last_name":"Sorichetti"},{"first_name":"Alice","full_name":"Cezanne, Alice","last_name":"Cezanne"},{"last_name":"Foo","full_name":"Foo, Sherman","first_name":"Sherman"},{"first_name":"Yin Wei","last_name":"Kuo","full_name":"Kuo, Yin Wei"},{"first_name":"Baukje","full_name":"Hoogenberg, Baukje","last_name":"Hoogenberg"},{"full_name":"Radoux-Mergault, Arthur","last_name":"Radoux-Mergault","first_name":"Arthur"},{"full_name":"Mawdesley, Eloise","last_name":"Mawdesley","first_name":"Eloise"},{"first_name":"Lydia Daniels","last_name":"Gatward","full_name":"Gatward, Lydia Daniels"},{"first_name":"Jerome","last_name":"Boulanger","full_name":"Boulanger, Jerome"},{"first_name":"Ulrike","last_name":"Schulze","full_name":"Schulze, Ulrike"},{"first_name":"Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","last_name":"Šarić","orcid":"0000-0002-7854-2139"},{"last_name":"Baum","full_name":"Baum, Buzz","first_name":"Buzz"}],"doi":"10.1073/pnas.2513939122","OA_type":"hybrid","language":[{"iso":"eng"}],"volume":122,"issue":"42","department":[{"_id":"AnSa"}],"publication_status":"published","intvolume":"       122","has_accepted_license":"1","scopus_import":"1","acknowledgement":"We thank Matthew Kenneth for his assistance with live cell imaging. We thank Arthur Charles-Orszag and Dyche Mullins for generously gifting the SegA and SegB antibodies, and Sonja-Verena Albers for gifting the CdvA-HA overexpression plasmid. We thank the Light Microscopy and Flow Cytometry facilities at the MRC-LMB, and all the core staff at the MRC-LMB for their support. We thank all members of the Baum lab for helpful discussions. We would like to thank Magdalena Lechowska, Gautam Dey, Laura Downie, and Iva Tolic for critical reading of the manuscript. J.P. was supported by the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27). A.C. was funded by an EMBO Postdoctoral fellowship (ALTF_1041-2021), a Marie Sklodowska-Curie Individual Fellowship (101068523) provided by UKRI and by the Wellcome Trust (222460/Z/21/Z). B.H. was supported by Wellcome Trust (203276/A/16/Z). Y.-W.K. was supported by an EMBO postdoctoral fellowship (ALTF 903-2021) and by the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27); S.F. was supported by the Wellcome Trust (222460/Z/21/Z); B.B. received support from the MRC LMB, the Wellcome Trust (203276/Z/16/Z) and (222460/Z/21/Z), the VW Foundation (94933), and from the Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems Initiative (9346). V.S. and A.Š. acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant no.802960 to A.Š.), the Vallee Scholarship, and the EMBO Young Investigator Programme (A.Š.). The collaborative work of A.Š.’s and B.B. teams was also supported by a Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation 735929LPI.","status":"public","file_date_updated":"2025-10-27T08:12:59Z","article_type":"original","PlanS_conform":"1","type":"journal_article","quality_controlled":"1","date_created":"2025-10-26T23:01:33Z","pmid":1,"_id":"20530","ddc":["570"],"ec_funded":1,"publication_identifier":{"eissn":["1091-6490"]},"month":"10","year":"2025","project":[{"call_identifier":"H2020","grant_number":"802960","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines"},{"_id":"349b6ff1-11ca-11ed-8bc3-f006047c2eeb","name":"EMBO Young Investigator Program - Andela Saric"}],"publisher":"National Academy of Sciences","publication":"Proceedings of the National Academy of Sciences of the United States of America","date_published":"2025-10-21T00:00:00Z","oa_version":"Published Version","page":"e2513939122","date_updated":"2025-10-27T08:14:15Z","article_processing_charge":"Yes (in subscription journal)","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"external_id":{"pmid":["41091768"]},"oa":1,"day":"21","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"relation":"main_file","checksum":"3555d51f438d2e356039a9b697eac3ee","date_updated":"2025-10-27T08:12:59Z","access_level":"open_access","date_created":"2025-10-27T08:12:59Z","content_type":"application/pdf","file_name":"2025_PNAS_Parham.pdf","success":1,"file_id":"20543","creator":"dernst","file_size":2649194}],"citation":{"ieee":"J. Parham <i>et al.</i>, “Temporal and spatial coordination of DNA segregation and cell division in an archaeon,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 122, no. 42. National Academy of Sciences, p. e2513939122, 2025.","ama":"Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination of DNA segregation and cell division in an archaeon. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2025;122(42):e2513939122. doi:<a href=\"https://doi.org/10.1073/pnas.2513939122\">10.1073/pnas.2513939122</a>","short":"J. Parham, V. Sorichetti, A. Cezanne, S. Foo, Y.W. Kuo, B. Hoogenberg, A. Radoux-Mergault, E. Mawdesley, L.D. Gatward, J. Boulanger, U. Schulze, A. Šarić, B. Baum, Proceedings of the National Academy of Sciences of the United States of America 122 (2025) e2513939122.","mla":"Parham, Joe, et al. “Temporal and Spatial Coordination of DNA Segregation and Cell Division in an Archaeon.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 122, no. 42, National Academy of Sciences, 2025, p. e2513939122, doi:<a href=\"https://doi.org/10.1073/pnas.2513939122\">10.1073/pnas.2513939122</a>.","ista":"Parham J, Sorichetti V, Cezanne A, Foo S, Kuo YW, Hoogenberg B, Radoux-Mergault A, Mawdesley E, Gatward LD, Boulanger J, Schulze U, Šarić A, Baum B. 2025. Temporal and spatial coordination of DNA segregation and cell division in an archaeon. Proceedings of the National Academy of Sciences of the United States of America. 122(42), e2513939122.","apa":"Parham, J., Sorichetti, V., Cezanne, A., Foo, S., Kuo, Y. W., Hoogenberg, B., … Baum, B. (2025). Temporal and spatial coordination of DNA segregation and cell division in an archaeon. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2513939122\">https://doi.org/10.1073/pnas.2513939122</a>","chicago":"Parham, Joe, Valerio Sorichetti, Alice Cezanne, Sherman Foo, Yin Wei Kuo, Baukje Hoogenberg, Arthur Radoux-Mergault, et al. “Temporal and Spatial Coordination of DNA Segregation and Cell Division in an Archaeon.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2513939122\">https://doi.org/10.1073/pnas.2513939122</a>."},"abstract":[{"text":"Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segregation and division are mechanistically coupled in archaeal relatives of eukaryotes, which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we first describe the series of sequential changes in DNA organization that accompany cell division in Sulfolobus, which computational modeling shows likely aid genome segregation. Through a perturbation analysis we identify a regulatory checkpoint which ensures that the compaction of the genome into two spatially segregated nucleoids only occurs once cells have assembled a division ring—which also defines the axis of DNA segregation. Finally, we show that DNA compaction and segregation depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence leads to bridging DNA. Taken together, these data show how regulatory checkpoints like those operating in eukaryotes aid high-fidelity division in an archaeon.","lang":"eng"}],"title":"Temporal and spatial coordination of DNA segregation and cell division in an archaeon","OA_place":"publisher"}