---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20530'
abstract:
- lang: eng
text: Cells must coordinate DNA segregation with cytokinesis to ensure that each
daughter cell inherits a complete genome. Here, we explore how DNA segregation
and division are mechanistically coupled in archaeal relatives of eukaryotes,
which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we
first describe the series of sequential changes in DNA organization that accompany
cell division in Sulfolobus, which computational modeling shows likely aid genome
segregation. Through a perturbation analysis we identify a regulatory checkpoint
which ensures that the compaction of the genome into two spatially segregated
nucleoids only occurs once cells have assembled a division ring—which also defines
the axis of DNA segregation. Finally, we show that DNA compaction and segregation
depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence
leads to bridging DNA. Taken together, these data show how regulatory checkpoints
like those operating in eukaryotes aid high-fidelity division in an archaeon.
acknowledgement: We thank Matthew Kenneth for his assistance with live cell imaging.
We thank Arthur Charles-Orszag and Dyche Mullins for generously gifting the SegA
and SegB antibodies, and Sonja-Verena Albers for gifting the CdvA-HA overexpression
plasmid. We thank the Light Microscopy and Flow Cytometry facilities at the MRC-LMB,
and all the core staff at the MRC-LMB for their support. We thank all members of
the Baum lab for helpful discussions. We would like to thank Magdalena Lechowska,
Gautam Dey, Laura Downie, and Iva Tolic for critical reading of the manuscript.
J.P. was supported by the Medical Research Council—Laboratory of Molecular Biology
(MC_UP_1201/27). A.C. was funded by an EMBO Postdoctoral fellowship (ALTF_1041-2021),
a Marie Sklodowska-Curie Individual Fellowship (101068523) provided by UKRI and
by the Wellcome Trust (222460/Z/21/Z). B.H. was supported by Wellcome Trust (203276/A/16/Z).
Y.-W.K. was supported by an EMBO postdoctoral fellowship (ALTF 903-2021) and by
the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27); S.F.
was supported by the Wellcome Trust (222460/Z/21/Z); B.B. received support from
the MRC LMB, the Wellcome Trust (203276/Z/16/Z) and (222460/Z/21/Z), the VW Foundation
(94933), and from the Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems
Initiative (9346). V.S. and A.Š. acknowledge funding from the European Research
Council under the European Union’s Horizon 2020 research and innovation programme
(grant no.802960 to A.Š.), the Vallee Scholarship, and the EMBO Young Investigator
Programme (A.Š.). The collaborative work of A.Š.’s and B.B. teams was also supported
by a Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation
735929LPI.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Joe
full_name: Parham, Joe
last_name: Parham
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Alice
full_name: Cezanne, Alice
last_name: Cezanne
- first_name: Sherman
full_name: Foo, Sherman
last_name: Foo
- first_name: Yin Wei
full_name: Kuo, Yin Wei
last_name: Kuo
- first_name: Baukje
full_name: Hoogenberg, Baukje
last_name: Hoogenberg
- first_name: Arthur
full_name: Radoux-Mergault, Arthur
last_name: Radoux-Mergault
- first_name: Eloise
full_name: Mawdesley, Eloise
last_name: Mawdesley
- first_name: Lydia Daniels
full_name: Gatward, Lydia Daniels
last_name: Gatward
- first_name: Jerome
full_name: Boulanger, Jerome
last_name: Boulanger
- first_name: Ulrike
full_name: Schulze, Ulrike
last_name: Schulze
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
- first_name: Buzz
full_name: Baum, Buzz
last_name: Baum
citation:
ama: Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination
of DNA segregation and cell division in an archaeon. Proceedings of the National
Academy of Sciences. 2025;122(42):e2513939122. doi:10.1073/pnas.2513939122
apa: Parham, J., Sorichetti, V., Cezanne, A., Foo, S., Kuo, Y. W., Hoogenberg, B.,
… Baum, B. (2025). Temporal and spatial coordination of DNA segregation and cell
division in an archaeon. Proceedings of the National Academy of Sciences.
National Academy of Sciences. https://doi.org/10.1073/pnas.2513939122
chicago: Parham, Joe, Valerio Sorichetti, Alice Cezanne, Sherman Foo, Yin Wei Kuo,
Baukje Hoogenberg, Arthur Radoux-Mergault, et al. “Temporal and Spatial Coordination
of DNA Segregation and Cell Division in an Archaeon.” Proceedings of the National
Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2513939122.
ieee: J. Parham et al., “Temporal and spatial coordination of DNA segregation
and cell division in an archaeon,” Proceedings of the National Academy of Sciences,
vol. 122, no. 42. National Academy of Sciences, p. e2513939122, 2025.
ista: Parham J, Sorichetti V, Cezanne A, Foo S, Kuo YW, Hoogenberg B, Radoux-Mergault
A, Mawdesley E, Gatward LD, Boulanger J, Schulze U, Šarić A, Baum B. 2025. Temporal
and spatial coordination of DNA segregation and cell division in an archaeon.
Proceedings of the National Academy of Sciences. 122(42), e2513939122.
mla: Parham, Joe, et al. “Temporal and Spatial Coordination of DNA Segregation and
Cell Division in an Archaeon.” Proceedings of the National Academy of Sciences,
vol. 122, no. 42, National Academy of Sciences, 2025, p. e2513939122, doi:10.1073/pnas.2513939122.
short: J. Parham, V. Sorichetti, A. Cezanne, S. Foo, Y.W. Kuo, B. Hoogenberg, A.
Radoux-Mergault, E. Mawdesley, L.D. Gatward, J. Boulanger, U. Schulze, A. Šarić,
B. Baum, Proceedings of the National Academy of Sciences 122 (2025) e2513939122.
date_created: 2025-10-26T23:01:33Z
date_published: 2025-10-21T00:00:00Z
date_updated: 2026-02-16T12:32:31Z
day: '21'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1073/pnas.2513939122
ec_funded: 1
external_id:
isi:
- '001620648600001'
pmid:
- '41091768'
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language:
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month: '10'
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page: e2513939122
pmid: 1
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
name: EMBO Young Investigator Program - Andela Saric
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
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status: public
title: Temporal and spatial coordination of DNA segregation and cell division in an
archaeon
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legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
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...