{"ddc":["580"],"OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","scopus_import":"1","status":"public","intvolume":"       122","oa":1,"PlanS_conform":"1","external_id":{"pmid":["40986351"]},"file_date_updated":"2025-11-24T13:48:09Z","tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"date_published":"2025-09-23T00:00:00Z","department":[{"_id":"JiFr"}],"volume":122,"publication_status":"published","project":[{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"date_created":"2025-11-12T10:03:20Z","article_processing_charge":"Yes (in subscription journal)","OA_type":"hybrid","publication":"Proceedings of the National Academy of Sciences of the United States of America","issue":"39","_id":"20635","doi":"10.1073/pnas.2512274122","year":"2025","language":[{"iso":"eng"}],"publisher":"National Academy of Sciences","oa_version":"Published Version","acknowledgement":"This research was funded by Biological Breeding-National Science and Technology Major Project (2023ZD0407201), China Postdoctoral Science Foundation (2024M763575), China Agricultural University Fund (2025RC042), Chinese Universities Scientific Fund (2024RC031), and Austrian Science Fund (FWF; I 6123-B).","article_type":"original","date_updated":"2025-11-24T13:49:12Z","page":"e2512274122","quality_controlled":"1","day":"23","pmid":1,"author":[{"first_name":"F","last_name":"Sheng","full_name":"Sheng, F"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"first_name":"Y","last_name":"Wang","full_name":"Wang, Y"},{"full_name":"Li, Y","last_name":"Li","first_name":"Y"},{"first_name":"JA","full_name":"Zhang, JA","last_name":"Zhang"},{"last_name":"Zhang","full_name":"Zhang, Z","first_name":"Z"},{"full_name":"Qin, X","last_name":"Qin","first_name":"X"},{"last_name":"Zhang","full_name":"Zhang, S","first_name":"S"},{"first_name":"W","last_name":"Song","full_name":"Song, W"},{"first_name":"J","full_name":"Li, J","last_name":"Li"},{"first_name":"Y","full_name":"Guo, Y","last_name":"Guo"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"full_name":"Gong, Z","last_name":"Gong","first_name":"Z"},{"first_name":"Q","last_name":"Zhang","full_name":"Zhang, Q"},{"full_name":"Zhang, J","last_name":"Zhang","first_name":"J"}],"abstract":[{"lang":"eng","text":"Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s-mediated phosphorylation modulates both the polar localization and transport activity of PIN2. Importantly, SnRK2s function antagonistically to the AGCVIII kinase PID, which phosphorylates PIN2 at a distinct site (S258), establishing a regulatory balance essential for adaptive root growth. Structural modeling and phosphorylation assays further suggest that SnRK2s-mediated phosphorylation at S259 sterically hinders access of PID to S258, providing a mechanistic basis for their antagonistic relationship. These findings uncover a novel regulatory mechanism, by which plants fine-tune root developmental programs to adapt to environmental stimuli, highlighting the evolutionary significance of multilayered kinase-mediated regulation in plant adaptation."}],"has_accepted_license":"1","month":"09","title":"Antagonistic SnRK2 and PID kinases' action on auxin transport-mediated root gravitropism","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"citation":{"ama":"Sheng F, Gao Y, Wang Y, et al. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2025;122(39):e2512274122. doi:<a href=\"https://doi.org/10.1073/pnas.2512274122\">10.1073/pnas.2512274122</a>","ieee":"F. Sheng <i>et al.</i>, “Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 122, no. 39. National Academy of Sciences, p. e2512274122, 2025.","ista":"Sheng F, Gao Y, Wang Y, Li Y, Zhang J, Zhang Z, Qin X, Zhang S, Song W, Li J, Guo Y, Friml J, Gong Z, Zhang Q, Zhang J. 2025. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences of the United States of America. 122(39), e2512274122.","apa":"Sheng, F., Gao, Y., Wang, Y., Li, Y., Zhang, J., Zhang, Z., … Zhang, J. (2025). Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2512274122\">https://doi.org/10.1073/pnas.2512274122</a>","chicago":"Sheng, F, Y Gao, Y Wang, Y Li, JA Zhang, Z Zhang, X Qin, et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2512274122\">https://doi.org/10.1073/pnas.2512274122</a>.","short":"F. Sheng, Y. Gao, Y. Wang, Y. Li, J. Zhang, Z. Zhang, X. Qin, S. Zhang, W. Song, J. Li, Y. Guo, J. Friml, Z. Gong, Q. Zhang, J. Zhang, Proceedings of the National Academy of Sciences of the United States of America 122 (2025) e2512274122.","mla":"Sheng, F., et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 122, no. 39, National Academy of Sciences, 2025, p. e2512274122, doi:<a href=\"https://doi.org/10.1073/pnas.2512274122\">10.1073/pnas.2512274122</a>."},"file":[{"date_created":"2025-11-24T13:48:09Z","checksum":"38b723a909bf321d7ee537c9d064aa25","date_updated":"2025-11-24T13:48:09Z","access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_id":"20681","creator":"dernst","file_name":"2025_PNAS_Sheng.pdf","file_size":2667764,"success":1}]}