{"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        40","status":"public","scopus_import":"1","ddc":["570"],"OA_place":"publisher","department":[{"_id":"RySh"}],"date_published":"2025-12-01T00:00:00Z","volume":40,"publication_status":"published","file_date_updated":"2025-11-24T10:23:39Z","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"PlanS_conform":"1","oa":1,"external_id":{"pmid":["40704638"]},"year":"2025","publisher":"Sercrisma International","language":[{"iso":"eng"}],"OA_type":"gold","article_processing_charge":"Yes","date_created":"2025-11-23T23:01:37Z","_id":"20659","issue":"12","doi":"10.14670/HH-18-970","publication":"Histology and Histopathology","has_accepted_license":"1","month":"12","title":"Developmental regulation of GABAB receptors and downstream molecules in the mouse brain","author":[{"first_name":"Carolina","full_name":"Aguado, Carolina","last_name":"Aguado"},{"last_name":"Alfaro-Ruiz","full_name":"Alfaro-Ruiz, Rocío","first_name":"Rocío"},{"first_name":"María Llanos","last_name":"Martínez-Poyato","full_name":"Martínez-Poyato, María Llanos"},{"first_name":"Ana Esther","full_name":"Moreno-Martínez, Ana Esther","last_name":"Moreno-Martínez"},{"full_name":"García-Madrona, Sebastián","last_name":"García-Madrona","first_name":"Sebastián"},{"first_name":"Alberto","last_name":"Roldán-Sastre","full_name":"Roldán-Sastre, Alberto"},{"last_name":"Alonso-Gómez","full_name":"Alonso-Gómez, Pablo","first_name":"Pablo"},{"first_name":"Miriam","full_name":"Fernández, Miriam","last_name":"Fernández"},{"first_name":"Ricardo","full_name":"Puertas-Avendaño, Ricardo","last_name":"Puertas-Avendaño"},{"last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Kirill A.","full_name":"Martemyanov, Kirill A.","last_name":"Martemyanov"},{"full_name":"Luján, Rafael","last_name":"Luján","first_name":"Rafael"}],"abstract":[{"lang":"eng","text":"Metabotropic GABA (GABAB) receptors have modulatory functions on neuronal excitability and\r\nneurotransmitter release. To fulfil these functions, GABAB receptors form macromolecular signaling complexes with G proteins, effectors, and other associated proteins. Here we investigated the postnatal development of GABAB receptors (GABAB1 and GABAB2 subunits) in mouse brain, focusing on potential similarities in the spatial and temporal expression pattern\r\nof their associated proteins CaV2.1, Gαo, Gβ5, and RGS7, using histoblots, immunofluorescence, and immunoelectron microscopic techniques. At all ages analyzed, histoblot showed that the six proteins were widely expressed in the brain, with mostly an\r\noverlapping pattern throughout postnatal development. In the hippocampus, immunoelectron microscopy and quantitative analysis of immunoparticles for GABAB1, GABAB2, Gαo, Gβ5, and RGS7 revealed their progressive enrichment around excitatory synapses on dendritic spines of CA1 pyramidal cells toward P15. At presynaptic sites, GABAB receptors colocalize with\r\nCaV2.1, Gαo, Gβ5, and RGS7 in the active zone and extrasynaptic membranes of axon terminals, establishing synapses on dendritic spines of CA1 pyramidal cells. In the cerebellum, double immunofluorescence at P7 and P10 revealed the colocalization of GABAB1 and CaV2.1\r\nin the whole dendritic tree of developing Purkinje cells. Immunoelectron microscopy at P15 showed that GABAB1, GABAB2, CaV2.1, Gαo, Gβ5, and RGS7 are distributed along the dendritic surface of Purkinje cells, enriched close to excitatory synapses in spines.\r\nAltogether, these data suggest that macromolecular complexes composed of GABAB1 /GABAB2/CaV2.1/ Gαo/Gβ5/RGS7 are pre-assembled during key stages of postnatal development in hippocampal and cerebellar neurons."}],"file":[{"success":1,"file_size":18093266,"file_name":"2025_HistologyHistopathology_Aguado.pdf","creator":"dernst","file_id":"20677","content_type":"application/pdf","relation":"main_file","access_level":"open_access","date_updated":"2025-11-24T10:23:39Z","checksum":"779d67434400c0f94af42c35ba21c672","date_created":"2025-11-24T10:23:39Z"}],"citation":{"apa":"Aguado, C., Alfaro-Ruiz, R., Martínez-Poyato, M. L., Moreno-Martínez, A. E., García-Madrona, S., Roldán-Sastre, A., … Luján, R. (2025). Developmental regulation of GABAB receptors and downstream molecules in the mouse brain. <i>Histology and Histopathology</i>. Sercrisma International. <a href=\"https://doi.org/10.14670/HH-18-970\">https://doi.org/10.14670/HH-18-970</a>","chicago":"Aguado, Carolina, Rocío Alfaro-Ruiz, María Llanos Martínez-Poyato, Ana Esther Moreno-Martínez, Sebastián García-Madrona, Alberto Roldán-Sastre, Pablo Alonso-Gómez, et al. “Developmental Regulation of GABAB Receptors and Downstream Molecules in the Mouse Brain.” <i>Histology and Histopathology</i>. Sercrisma International, 2025. <a href=\"https://doi.org/10.14670/HH-18-970\">https://doi.org/10.14670/HH-18-970</a>.","mla":"Aguado, Carolina, et al. “Developmental Regulation of GABAB Receptors and Downstream Molecules in the Mouse Brain.” <i>Histology and Histopathology</i>, vol. 40, no. 12, Sercrisma International, 2025, pp. 1967–84, doi:<a href=\"https://doi.org/10.14670/HH-18-970\">10.14670/HH-18-970</a>.","short":"C. Aguado, R. Alfaro-Ruiz, M.L. Martínez-Poyato, A.E. Moreno-Martínez, S. García-Madrona, A. Roldán-Sastre, P. Alonso-Gómez, M. Fernández, R. Puertas-Avendaño, R. Shigemoto, K.A. Martemyanov, R. Luján, Histology and Histopathology 40 (2025) 1967–1984.","ama":"Aguado C, Alfaro-Ruiz R, Martínez-Poyato ML, et al. Developmental regulation of GABAB receptors and downstream molecules in the mouse brain. <i>Histology and Histopathology</i>. 2025;40(12):1967-1984. doi:<a href=\"https://doi.org/10.14670/HH-18-970\">10.14670/HH-18-970</a>","ieee":"C. Aguado <i>et al.</i>, “Developmental regulation of GABAB receptors and downstream molecules in the mouse brain,” <i>Histology and Histopathology</i>, vol. 40, no. 12. Sercrisma International, pp. 1967–1984, 2025.","ista":"Aguado C, Alfaro-Ruiz R, Martínez-Poyato ML, Moreno-Martínez AE, García-Madrona S, Roldán-Sastre A, Alonso-Gómez P, Fernández M, Puertas-Avendaño R, Shigemoto R, Martemyanov KA, Luján R. 2025. Developmental regulation of GABAB receptors and downstream molecules in the mouse brain. Histology and Histopathology. 40(12), 1967–1984."},"publication_identifier":{"eissn":["1699-5848"]},"date_updated":"2025-11-24T10:24:35Z","article_type":"original","page":"1967-1984","acknowledgement":"We thank Ms. Diane Latawiec for the English revision of the manuscript. Funding sources were the Spanish Ministerio de Economía y Competitividad, and Junta de Comunidades de CastillaLa Mancha (Spain). Grants PID2021-125875OB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and “ERDF A way of making Europe” to RL. This work was also supported by Castilla-La Mancha Regional Government and the European Regional Development Fund (SBPLY/21/180501/000064) and the Universidad de Castilla-La Mancha (2023-GRIN-34187) to RL.","quality_controlled":"1","oa_version":"Published Version","pmid":1,"day":"01"}