---
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20659'
abstract:
- lang: eng
  text: "Metabotropic GABA (GABAB) receptors have modulatory functions on neuronal
    excitability and\r\nneurotransmitter release. To fulfil these functions, GABAB
    receptors form macromolecular signaling complexes with G proteins, effectors,
    and other associated proteins. Here we investigated the postnatal development
    of GABAB receptors (GABAB1 and GABAB2 subunits) in mouse brain, focusing on potential
    similarities in the spatial and temporal expression pattern\r\nof their associated
    proteins CaV2.1, Gαo, Gβ5, and RGS7, using histoblots, immunofluorescence, and
    immunoelectron microscopic techniques. At all ages analyzed, histoblot showed
    that the six proteins were widely expressed in the brain, with mostly an\r\noverlapping
    pattern throughout postnatal development. In the hippocampus, immunoelectron microscopy
    and quantitative analysis of immunoparticles for GABAB1, GABAB2, Gαo, Gβ5, and
    RGS7 revealed their progressive enrichment around excitatory synapses on dendritic
    spines of CA1 pyramidal cells toward P15. At presynaptic sites, GABAB receptors
    colocalize with\r\nCaV2.1, Gαo, Gβ5, and RGS7 in the active zone and extrasynaptic
    membranes of axon terminals, establishing synapses on dendritic spines of CA1
    pyramidal cells. In the cerebellum, double immunofluorescence at P7 and P10 revealed
    the colocalization of GABAB1 and CaV2.1\r\nin the whole dendritic tree of developing
    Purkinje cells. Immunoelectron microscopy at P15 showed that GABAB1, GABAB2, CaV2.1,
    Gαo, Gβ5, and RGS7 are distributed along the dendritic surface of Purkinje cells,
    enriched close to excitatory synapses in spines.\r\nAltogether, these data suggest
    that macromolecular complexes composed of GABAB1 /GABAB2/CaV2.1/ Gαo/Gβ5/RGS7
    are pre-assembled during key stages of postnatal development in hippocampal and
    cerebellar neurons."
acknowledgement: We thank Ms. Diane Latawiec for the English revision of the manuscript.
  Funding sources were the Spanish Ministerio de Economía y Competitividad, and Junta
  de Comunidades de CastillaLa Mancha (Spain). Grants PID2021-125875OB-I00 funded
  by MCIN/AEI/ 10.13039/501100011033 and “ERDF A way of making Europe” to RL. This
  work was also supported by Castilla-La Mancha Regional Government and the European
  Regional Development Fund (SBPLY/21/180501/000064) and the Universidad de Castilla-La
  Mancha (2023-GRIN-34187) to RL.
article_processing_charge: Yes
article_type: original
author:
- first_name: Carolina
  full_name: Aguado, Carolina
  last_name: Aguado
- first_name: Rocío
  full_name: Alfaro-Ruiz, Rocío
  last_name: Alfaro-Ruiz
- first_name: María Llanos
  full_name: Martínez-Poyato, María Llanos
  last_name: Martínez-Poyato
- first_name: Ana Esther
  full_name: Moreno-Martínez, Ana Esther
  last_name: Moreno-Martínez
- first_name: Sebastián
  full_name: García-Madrona, Sebastián
  last_name: García-Madrona
- first_name: Alberto
  full_name: Roldán-Sastre, Alberto
  last_name: Roldán-Sastre
- first_name: Pablo
  full_name: Alonso-Gómez, Pablo
  last_name: Alonso-Gómez
- first_name: Miriam
  full_name: Fernández, Miriam
  last_name: Fernández
- first_name: Ricardo
  full_name: Puertas-Avendaño, Ricardo
  last_name: Puertas-Avendaño
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Kirill A.
  full_name: Martemyanov, Kirill A.
  last_name: Martemyanov
- first_name: Rafael
  full_name: Luján, Rafael
  last_name: Luján
citation:
  ama: Aguado C, Alfaro-Ruiz R, Martínez-Poyato ML, et al. Developmental regulation
    of GABAB receptors and downstream molecules in the mouse brain. <i>Histology and
    Histopathology</i>. 2025;40(12):1967-1984. doi:<a href="https://doi.org/10.14670/HH-18-970">10.14670/HH-18-970</a>
  apa: Aguado, C., Alfaro-Ruiz, R., Martínez-Poyato, M. L., Moreno-Martínez, A. E.,
    García-Madrona, S., Roldán-Sastre, A., … Luján, R. (2025). Developmental regulation
    of GABAB receptors and downstream molecules in the mouse brain. <i>Histology and
    Histopathology</i>. Sercrisma International. <a href="https://doi.org/10.14670/HH-18-970">https://doi.org/10.14670/HH-18-970</a>
  chicago: Aguado, Carolina, Rocío Alfaro-Ruiz, María Llanos Martínez-Poyato, Ana
    Esther Moreno-Martínez, Sebastián García-Madrona, Alberto Roldán-Sastre, Pablo
    Alonso-Gómez, et al. “Developmental Regulation of GABAB Receptors and Downstream
    Molecules in the Mouse Brain.” <i>Histology and Histopathology</i>. Sercrisma
    International, 2025. <a href="https://doi.org/10.14670/HH-18-970">https://doi.org/10.14670/HH-18-970</a>.
  ieee: C. Aguado <i>et al.</i>, “Developmental regulation of GABAB receptors and
    downstream molecules in the mouse brain,” <i>Histology and Histopathology</i>,
    vol. 40, no. 12. Sercrisma International, pp. 1967–1984, 2025.
  ista: Aguado C, Alfaro-Ruiz R, Martínez-Poyato ML, Moreno-Martínez AE, García-Madrona
    S, Roldán-Sastre A, Alonso-Gómez P, Fernández M, Puertas-Avendaño R, Shigemoto
    R, Martemyanov KA, Luján R. 2025. Developmental regulation of GABAB receptors
    and downstream molecules in the mouse brain. Histology and Histopathology. 40(12),
    1967–1984.
  mla: Aguado, Carolina, et al. “Developmental Regulation of GABAB Receptors and Downstream
    Molecules in the Mouse Brain.” <i>Histology and Histopathology</i>, vol. 40, no.
    12, Sercrisma International, 2025, pp. 1967–84, doi:<a href="https://doi.org/10.14670/HH-18-970">10.14670/HH-18-970</a>.
  short: C. Aguado, R. Alfaro-Ruiz, M.L. Martínez-Poyato, A.E. Moreno-Martínez, S.
    García-Madrona, A. Roldán-Sastre, P. Alonso-Gómez, M. Fernández, R. Puertas-Avendaño,
    R. Shigemoto, K.A. Martemyanov, R. Luján, Histology and Histopathology 40 (2025)
    1967–1984.
date_created: 2025-11-23T23:01:37Z
date_published: 2025-12-01T00:00:00Z
date_updated: 2025-11-24T10:24:35Z
day: '01'
ddc:
- '570'
department:
- _id: RySh
doi: 10.14670/HH-18-970
external_id:
  pmid:
  - '40704638'
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has_accepted_license: '1'
intvolume: '        40'
issue: '12'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 1967-1984
pmid: 1
publication: Histology and Histopathology
publication_identifier:
  eissn:
  - 1699-5848
publication_status: published
publisher: Sercrisma International
quality_controlled: '1'
scopus_import: '1'
status: public
title: Developmental regulation of GABAB receptors and downstream molecules in the
  mouse brain
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 40
year: '2025'
...