---
res:
  bibo_abstract:
  - In all domains of life, tRNAs mediate the transfer of genetic information from
    mRNAs to proteins. As their depletion suppresses translation and, consequently,
    viral replication, tRNAs represent long-standing and increasingly recognized targets
    of innate immunity1,2,3,4,5. Here we report Cas12a3 effector nucleases from type V
    CRISPR–Cas adaptive immune systems in bacteria that preferentially cleave tRNAs
    after recognition of target RNA. Cas12a3 orthologues belong to one of two previously
    unreported nuclease clades that exhibit RNA-mediated cleavage of non-target RNA,
    and are distinct from all other known type V systems. Through cell-based and biochemical
    assays and direct RNA sequencing, we demonstrate that recognition of a complementary
    target RNA by the CRISPR RNA triggers Cas12a3 to cleave the conserved 5′-CCA-3′
    tail of diverse tRNAs to drive growth arrest and anti-phage defence. Cryogenic
    electron microscopy structures further revealed a distinct tRNA-loading domain
    that positions the tRNA tail in the RuvC active site of the nuclease. By designing
    synthetic reporters that mimic the tRNA acceptor stem and tail, we expanded the
    capacity of current CRISPR-based diagnostics for multiplexed RNA detection. Overall,
    these findings reveal widespread tRNA inactivation as a previously unrecognized
    CRISPR-based immune strategy that broadens the application space of the existing
    CRISPR toolbox.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Oleg
      foaf_name: Dmytrenko, Oleg
      foaf_surname: Dmytrenko
  - foaf_Person:
      foaf_givenName: Biao
      foaf_name: Yuan, Biao
      foaf_surname: Yuan
  - foaf_Person:
      foaf_givenName: Kadin T.
      foaf_name: Crosby, Kadin T.
      foaf_surname: Crosby
  - foaf_Person:
      foaf_givenName: Max
      foaf_name: Krebel, Max
      foaf_surname: Krebel
  - foaf_Person:
      foaf_givenName: Xiye
      foaf_name: Chen, Xiye
      foaf_surname: Chen
  - foaf_Person:
      foaf_givenName: Jakub S.
      foaf_name: Nowak, Jakub S.
      foaf_surname: Nowak
  - foaf_Person:
      foaf_givenName: Andrzej
      foaf_name: Chramiec-Głąbik, Andrzej
      foaf_surname: Chramiec-Głąbik
  - foaf_Person:
      foaf_givenName: Bamidele
      foaf_name: Filani, Bamidele
      foaf_surname: Filani
  - foaf_Person:
      foaf_givenName: Anne-Sophie
      foaf_name: Gribling-Burrer, Anne-Sophie
      foaf_surname: Gribling-Burrer
  - foaf_Person:
      foaf_givenName: Wiep
      foaf_name: van der Toorn, Wiep
      foaf_surname: van der Toorn
  - foaf_Person:
      foaf_givenName: Max
      foaf_name: von Kleist, Max
      foaf_surname: von Kleist
  - foaf_Person:
      foaf_givenName: Tatjana
      foaf_name: Achmedov, Tatjana
      foaf_surname: Achmedov
  - foaf_Person:
      foaf_givenName: Redmond P.
      foaf_name: Smyth, Redmond P.
      foaf_surname: Smyth
  - foaf_Person:
      foaf_givenName: Sebastian
      foaf_name: Glatt, Sebastian
      foaf_surname: Glatt
  - foaf_Person:
      foaf_givenName: Jack Peter Kelly
      foaf_name: Bravo, Jack Peter Kelly
      foaf_surname: Bravo
      foaf_workInfoHomepage: http://www.librecat.org/personId=96aecfa5-8931-11ee-af30-aa6a5d6eee0e
    orcid: 0000-0003-0456-0753
  - foaf_Person:
      foaf_givenName: Dirk W.
      foaf_name: Heinz, Dirk W.
      foaf_surname: Heinz
  - foaf_Person:
      foaf_givenName: Ryan N.
      foaf_name: Jackson, Ryan N.
      foaf_surname: Jackson
  - foaf_Person:
      foaf_givenName: Chase L.
      foaf_name: Beisel, Chase L.
      foaf_surname: Beisel
  bibo_doi: 10.1038/s41586-025-09852-9
  bibo_volume: 649
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0028-0836
  - http://id.crossref.org/issn/1476-4687
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity@
  fabio_hasPubmedId: '41501459'
...