{"oa":1,"date_created":"2026-01-13T14:07:58Z","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","date_updated":"2026-01-14T10:54:31Z","doi":"10.1101/2025.01.06.631460","language":[{"iso":"eng"}],"OA_place":"repository","department":[{"_id":"JiFr"}],"corr_author":"1","day":"16","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","publication_status":"draft","citation":{"mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>.","ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, <a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","ieee":"I. Kulich <i>et al.</i>, “Armadillo repeat only proteins are required for the function of plant CNGC channels,” <i>bioRxiv</i>. .","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>","apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>"},"related_material":{"record":[{"status":"for_moderation","relation":"dissertation_contains","id":"20964"}]},"month":"05","article_processing_charge":"No","main_file_link":[{"url":"https://doi.org/10.1101/2025.01.06.631460","open_access":"1"}],"abstract":[{"lang":"eng","text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants."}],"type":"preprint","_id":"20982","author":[{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","first_name":"Ivan","full_name":"Kulich, Ivan","last_name":"Kulich"},{"last_name":"Oulehlová","first_name":"Denisa","full_name":"Oulehlová, Denisa"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev"},{"full_name":"Zou, Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia","last_name":"Zou"},{"full_name":"Lileikyte, Edita","first_name":"Edita","last_name":"Lileikyte"},{"last_name":"Bondar","full_name":"Bondar, Alexey","first_name":"Alexey"},{"full_name":"Kulichová, Katarína","first_name":"Katarína","last_name":"Kulichová"},{"last_name":"Janda","full_name":"Janda, Martin","first_name":"Martin"},{"full_name":"Iakovenko, Oksana","first_name":"Oksana","last_name":"Iakovenko"},{"last_name":"Neubergerová","full_name":"Neubergerová, Michaela","first_name":"Michaela"},{"first_name":"Tanja","full_name":"Studtrucker, Tanja","last_name":"Studtrucker"},{"full_name":"Pleskot, Roman","first_name":"Roman","last_name":"Pleskot"},{"first_name":"Petra","full_name":"Dietrich, Petra","last_name":"Dietrich"},{"last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas","orcid":"0000-0002-9767-8699","full_name":"Fendrych, Matyas"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2025","publication":"bioRxiv","tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"oa_version":"Preprint","title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","status":"public","date_published":"2025-05-16T00:00:00Z"}