{"day":"01","type":"journal_article","intvolume":"        69","publisher":"International Union of Crystallography","issue":"3","status":"public","date_updated":"2026-02-23T08:56:25Z","volume":69,"has_accepted_license":"1","publication_status":"published","OA_type":"closed access","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"313-315","year":"2013","publication_identifier":{"issn":["1744-3091"]},"doi":"10.1107/s1744309113003667","language":[{"iso":"eng"}],"publication":"Acta Crystallographica Section F Structural Biology Communications","date_created":"2026-01-29T22:11:18Z","_id":"21110","author":[{"last_name":"Aggarwal","full_name":"Aggarwal, Nidhi","first_name":"Nidhi"},{"first_name":"Pradeep K","orcid":"0000-0001-5996-956X","full_name":"Mandal, Pradeep K","last_name":"Mandal","id":"6a3def15-d4b4-11ef-9fa9-a24c1f545ec3"},{"last_name":"Gautham","first_name":"Namasivayam","full_name":"Gautham, Namasivayam"},{"last_name":"Chadha","first_name":"Anju","full_name":"Chadha, Anju"}],"quality_controlled":"1","oa_version":"None","month":"03","article_type":"original","article_processing_charge":"No","title":"Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330","extern":"1","date_published":"2013-03-01T00:00:00Z","abstract":[{"text":"The NAD(P)H-dependent carbonyl reductase from Candida parapsilosis ATCC 7330 catalyses the asymmetric reduction of ethyl 4-phenyl-2-oxobutanoate to ethyl (R)-4-phenyl-2-hydroxybutanoate, a precursor of angiotensin-converting enzyme inhibitors such as Cilazapril and Benazepril. The carbonyl reductase was expressed in Escherichia coli and purified by GST-affinity and size-exclusion chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.86 Å resolution. The asymmetric unit contained two molecules of carbonyl reductase, with a solvent content of 48%. The structure was solved by molecular replacement using cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae as a search model.","lang":"eng"}],"citation":{"apa":"Aggarwal, N., Mandal, P. K., Gautham, N., &#38; Chadha, A. (2013). Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330. <i>Acta Crystallographica Section F Structural Biology Communications</i>. International Union of Crystallography. <a href=\"https://doi.org/10.1107/s1744309113003667\">https://doi.org/10.1107/s1744309113003667</a>","short":"N. Aggarwal, P.K. Mandal, N. Gautham, A. Chadha, Acta Crystallographica Section F Structural Biology Communications 69 (2013) 313–315.","ama":"Aggarwal N, Mandal PK, Gautham N, Chadha A. Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330. <i>Acta Crystallographica Section F Structural Biology Communications</i>. 2013;69(3):313-315. doi:<a href=\"https://doi.org/10.1107/s1744309113003667\">10.1107/s1744309113003667</a>","mla":"Aggarwal, Nidhi, et al. “Expression, Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Carbonyl Reductase from Candida Parapsilosis ATCC 7330.” <i>Acta Crystallographica Section F Structural Biology Communications</i>, vol. 69, no. 3, International Union of Crystallography, 2013, pp. 313–15, doi:<a href=\"https://doi.org/10.1107/s1744309113003667\">10.1107/s1744309113003667</a>.","ieee":"N. Aggarwal, P. K. Mandal, N. Gautham, and A. Chadha, “Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330,” <i>Acta Crystallographica Section F Structural Biology Communications</i>, vol. 69, no. 3. International Union of Crystallography, pp. 313–315, 2013.","ista":"Aggarwal N, Mandal PK, Gautham N, Chadha A. 2013. Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330. Acta Crystallographica Section F Structural Biology Communications. 69(3), 313–315.","chicago":"Aggarwal, Nidhi, Pradeep K Mandal, Namasivayam Gautham, and Anju Chadha. “Expression, Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Carbonyl Reductase from Candida Parapsilosis ATCC 7330.” <i>Acta Crystallographica Section F Structural Biology Communications</i>. International Union of Crystallography, 2013. <a href=\"https://doi.org/10.1107/s1744309113003667\">https://doi.org/10.1107/s1744309113003667</a>."}}