---
OA_place: repository
OA_type: free access
_id: '21284'
abstract:
- lang: eng
  text: The advantageous characteristics attributed to the 19F nucleus have made it
    a popular target for NMR once again in recent years. Aside from solution NMR,
    an increasing number of studies have been conducted applying solid-state magic-angle-spinning
    NMR to fluorine-labeled samples. Here, the high chemical shift anisotropy and
    strong dipolar couplings can be utilized to get structural insights into proteins
    and measure long distances. Despite increasing popularity and promising benefits,
    the sensitivity of biomolecular 19F MAS NMR often suffers from slow longitudinal
    T1 relaxation and therefore long recycle delays. In this work, we expand paramagnetic
    doping, an approach commonly used to reduce proton T1 relaxation times, to 19F-labeled
    biological samples. We study the effect of Gd(DTPA) and Gd(DTPA-BMA) on 19F and
    13C T1 and T2 relaxation in a [5-19F13C]-tryptophan-labeled protein via 19F-detected
    MAS NMR experiments. The observed paramagnetic relaxation enhancement substantially
    reduces measurement times of 19F MAS NMR experiments without compromising resolution.
    Additionally, we report the chemical-shift assignments of all four fluorotryptophan
    signals in the 12 × 39 kDa large protein using a mutagenesis approach.
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: We thank Ben P. Tatman for insightful discussions. This research
  was supported by the Scientific Service Units (SSU) of Institute of Science and
  Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance
  Facility and the Lab Support Facility.
article_processing_charge: No
author:
- first_name: Lea Marie
  full_name: Becker, Lea Marie
  id: 36336939-eb97-11eb-a6c2-c83f1214ca79
  last_name: Becker
  orcid: 0000-0002-6401-5151
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
citation:
  ama: Becker LM, Schanda P. Research data for “Accelerated 19F biomolecular magic-angle
    spinning NMR with paramagnetic dopants.” 2026. doi:<a href="https://doi.org/10.15479/AT-ISTA-21284">10.15479/AT-ISTA-21284</a>
  apa: Becker, L. M., &#38; Schanda, P. (2026). Research data for “Accelerated 19F
    biomolecular magic-angle spinning NMR with paramagnetic dopants.” Institute of
    Science and Technology Austria. <a href="https://doi.org/10.15479/AT-ISTA-21284">https://doi.org/10.15479/AT-ISTA-21284</a>
  chicago: Becker, Lea Marie, and Paul Schanda. “Research Data for ‘Accelerated 19F
    Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.’” Institute of
    Science and Technology Austria, 2026. <a href="https://doi.org/10.15479/AT-ISTA-21284">https://doi.org/10.15479/AT-ISTA-21284</a>.
  ieee: L. M. Becker and P. Schanda, “Research data for ‘Accelerated 19F biomolecular
    magic-angle spinning NMR with paramagnetic dopants.’” Institute of Science and
    Technology Austria, 2026.
  ista: Becker LM, Schanda P. 2026. Research data for ‘Accelerated 19F biomolecular
    magic-angle spinning NMR with paramagnetic dopants’, Institute of Science and
    Technology Austria, <a href="https://doi.org/10.15479/AT-ISTA-21284">10.15479/AT-ISTA-21284</a>.
  mla: Becker, Lea Marie, and Paul Schanda. <i>Research Data for “Accelerated 19F
    Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.”</i> Institute
    of Science and Technology Austria, 2026, doi:<a href="https://doi.org/10.15479/AT-ISTA-21284">10.15479/AT-ISTA-21284</a>.
  short: L.M. Becker, P. Schanda, (2026).
contributor:
- contributor_type: researcher
  first_name: Giorgia
  id: 334a5e40-8747-11f0-b671-ba1f5154b4b4
  last_name: Toscano
- contributor_type: researcher
  first_name: Anna
  id: 9fb2a840-89e1-11ee-a8b7-cc5c7ba62471
  last_name: Kapitonova
- contributor_type: researcher
  first_name: Rajkumar
  id: a3089acd-6806-11ee-bacc-f0c7d500ad20
  last_name: Singh
- contributor_type: researcher
  first_name: Undina
  id: bb74f472-ae54-11eb-9835-bc9c22fb1183
  last_name: Guillerm
- contributor_type: researcher
  first_name: Roman
  last_name: Lichtenecker
corr_author: '1'
date_created: 2026-02-17T10:17:14Z
date_published: 2026-02-18T00:00:00Z
date_updated: 2026-02-18T10:12:49Z
day: '18'
ddc:
- '541'
department:
- _id: GradSch
- _id: PaSc
doi: 10.15479/AT-ISTA-21284
file:
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  date_created: 2026-02-17T10:11:14Z
  date_updated: 2026-02-17T10:11:14Z
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  checksum: e24aebcdb8856cb181cbaa02de020ddb
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  date_created: 2026-02-17T10:11:14Z
  date_updated: 2026-02-17T10:11:14Z
  file_id: '21286'
  file_name: README.txt
  file_size: 1993
  relation: table_of_contents
file_date_updated: 2026-02-17T10:11:14Z
has_accepted_license: '1'
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '2'
oa: 1
oa_version: None
publisher: Institute of Science and Technology Austria
status: public
title: Research data for "Accelerated 19F biomolecular magic-angle spinning NMR with
  paramagnetic dopants"
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2026'
...