{"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"title":"Lineage origin of spinal cord cell type diversity","article_processing_charge":"No","oa_version":"Preprint","day":"16","date_published":"2026-02-16T00:00:00Z","year":"2026","doi":"10.64898/2026.02.12.705305","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.02.12.705305"}],"OA_place":"repository","publication_status":"submitted","type":"preprint","author":[{"last_name":"Gobeil","id":"2f3e9efb-eb24-11ec-86b2-88efb11d59fa","full_name":"Gobeil, Sophie A","first_name":"Sophie A"},{"first_name":"Francisco","full_name":"Da Silveira Neto, Francisco","id":"8cfb7412-10a7-11f1-add1-82b44e6418f2","last_name":"Da Silveira Neto"},{"first_name":"Giulia","full_name":"Silvestrelli, Giulia","last_name":"Silvestrelli","id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9"},{"id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","last_name":"Smits","full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert"},{"first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher"},{"last_name":"Cheung","id":"471195F6-F248-11E8-B48F-1D18A9856A87","first_name":"Giselle T","orcid":"0000-0001-8457-2572","full_name":"Cheung, Giselle T"},{"first_name":"Simon","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney","orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger"}],"date_updated":"2026-02-23T11:09:15Z","citation":{"chicago":"Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B. Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>.","ista":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity. bioRxiv, <a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","short":"S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher, G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).","ieee":"S. A. Gobeil <i>et al.</i>, “Lineage origin of spinal cord cell type diversity,” <i>bioRxiv</i>. .","ama":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>","apa":"Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher, C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>","mla":"Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>."},"acknowledgement":"We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko, and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB consortium for comments on\r\nthe manuscript. We are also grateful for the technical support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA). In addition, we thank our funding sources for providing\r\nthe resources to do these experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.); Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C. by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S. and G.C. were\r\nadditionally supported by institutional funds from the ISTA and the University of Exeter,\r\nrespectively. ","OA_type":"green","status":"public","project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e"},{"_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"}],"abstract":[{"text":"The complexity and specificity of movement in vertebrates is driven by a rich diversity of spinal motor and interneuron cell types. During development, eleven spinal cord progenitor domains generate an equivalent number of cardinal neuron types. How progenitor domains, individual progenitors, and post-mitotic diversity relate is still unknown. We performed high-resolution, single-progenitor cell lineage tracing in the embryonic mouse spinal cord using mosaic analysis with double markers (MADM). Our quantitative study of lineage progression revealed that spinal cord progenitors undergo highly variable numbers of proliferative, neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons migrate radially over large distances, span the dorsoventral axis, and even cross the midline, demonstrating striking bilaterality. Molecular and morphometric analysis indicate high levels of progenitor multipotency, with an individual progenitor capable of producing several molecularly and morphologically distinct neuron types, as well as astrocytes. These findings redefine spinal cord development as a process in which lineage variability — rather than rigid progenitor identity — drives the generation of cellular diversity.","lang":"eng"}],"has_accepted_license":"1","month":"02","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"oa":1,"ddc":["570"],"date_created":"2026-02-17T11:36:20Z","department":[{"_id":"SiHi"},{"_id":"LoSw"}],"_id":"21291","publication":"bioRxiv"}