---
res:
  bibo_abstract:
  - 'Background: Low-volume trapping columns are essential for sample enrichment,
    desalting, and injection profile focusing on nano-LC–MS-based proteomics. They
    enable higher sample loading, improve chromatographic performance, and protect
    the analytical column by removing salts and contaminants. Recently, monolithic
    trap columns with micropillar architecture have emerged as alternatives to conventionally
    packed traps. This study compares the performance of a packed and a micropillar
    monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic
    digest of HeLa cell lysate was analyzed under identical LC–MS conditions using
    both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on
    a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database
    (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities
    was performed in Perseus using Welch’s t-test and clustering analysis. Results:
    Over 2500 proteins were identified with both setups. The packed trap column yielded
    more total peptides, particularly those with post-translational modifications
    and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate
    hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping
    efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column
    design significantly influences peptide recovery and proteome coverage.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jadranka
      foaf_name: Miletić Vukajlović, Jadranka
      foaf_surname: Miletić Vukajlović
  - foaf_Person:
      foaf_givenName: Bojana
      foaf_name: Ilić, Bojana
      foaf_surname: Ilić
  - foaf_Person:
      foaf_givenName: Bella
      foaf_name: Bruszel, Bella
      foaf_surname: Bruszel
      foaf_workInfoHomepage: http://www.librecat.org/personId=70abbbb3-88ea-11ec-8e0a-e8c939944834
  - foaf_Person:
      foaf_givenName: Tanja
      foaf_name: Panić-Janković, Tanja
      foaf_surname: Panić-Janković
  - foaf_Person:
      foaf_givenName: Goran
      foaf_name: Mitulović, Goran
      foaf_surname: Mitulović
  bibo_doi: 10.3390/proteomes14010010
  bibo_issue: '1'
  bibo_volume: 14
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2227-7382
  dct_language: eng
  dct_publisher: MDPI@
  dct_title: Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses@
  fabio_hasPubmedId: '41893725'
...