---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21711'
abstract:
- lang: eng
  text: 'Background: Low-volume trapping columns are essential for sample enrichment,
    desalting, and injection profile focusing on nano-LC–MS-based proteomics. They
    enable higher sample loading, improve chromatographic performance, and protect
    the analytical column by removing salts and contaminants. Recently, monolithic
    trap columns with micropillar architecture have emerged as alternatives to conventionally
    packed traps. This study compares the performance of a packed and a micropillar
    monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic
    digest of HeLa cell lysate was analyzed under identical LC–MS conditions using
    both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on
    a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database
    (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities
    was performed in Perseus using Welch’s t-test and clustering analysis. Results:
    Over 2500 proteins were identified with both setups. The packed trap column yielded
    more total peptides, particularly those with post-translational modifications
    and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate
    hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping
    efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column
    design significantly influences peptide recovery and proteome coverage.'
acknowledgement: 'The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel
  Nicolas, Institute for Science and Technology Austria, for their support. This research
  was conducted during a student residency in Vienna under the auspices of OeAD. ZI:
  ICM-2016-03196.'
article_number: '10'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jadranka
  full_name: Miletić Vukajlović, Jadranka
  last_name: Miletić Vukajlović
- first_name: Bojana
  full_name: Ilić, Bojana
  last_name: Ilić
- first_name: Bella
  full_name: Bruszel, Bella
  id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
  last_name: Bruszel
- first_name: Tanja
  full_name: Panić-Janković, Tanja
  last_name: Panić-Janković
- first_name: Goran
  full_name: Mitulović, Goran
  last_name: Mitulović
citation:
  ama: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison
    of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar
    trap columns for proteomics analyses. <i>Proteomes</i>. 2026;14(1). doi:<a href="https://doi.org/10.3390/proteomes14010010">10.3390/proteomes14010010</a>
  apa: Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., &#38; Mitulović,
    G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. MDPI.
    <a href="https://doi.org/10.3390/proteomes14010010">https://doi.org/10.3390/proteomes14010010</a>
  chicago: Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković,
    and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides
    on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>.
    MDPI, 2026. <a href="https://doi.org/10.3390/proteomes14010010">https://doi.org/10.3390/proteomes14010010</a>.
  ieee: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović,
    “Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses,” <i>Proteomes</i>, vol.
    14, no. 1. MDPI, 2026.
  ista: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026.
    Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.
  mla: Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency
    for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics
    Analyses.” <i>Proteomes</i>, vol. 14, no. 1, 10, MDPI, 2026, doi:<a href="https://doi.org/10.3390/proteomes14010010">10.3390/proteomes14010010</a>.
  short: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović,
    Proteomes 14 (2026).
date_created: 2026-04-12T22:01:49Z
date_published: 2026-03-01T00:00:00Z
date_updated: 2026-05-04T10:36:21Z
day: '01'
ddc:
- '540'
department:
- _id: MassSpec
doi: 10.3390/proteomes14010010
external_id:
  pmid:
  - '41893725'
file:
- access_level: open_access
  checksum: 1e0c66bbf4b6e0be626a8639ea664b63
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-04T10:31:35Z
  date_updated: 2026-05-04T10:31:35Z
  file_id: '21790'
  file_name: 2026_Proteomes_Vukajlovic.pdf
  file_size: 1009723
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T10:31:35Z
has_accepted_license: '1'
intvolume: '        14'
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proteomes
publication_identifier:
  eissn:
  - 2227-7382
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Comparison of the trapping efficiency for tryptic peptides on particle-packed
  and micro-pillar trap columns for proteomics analyses
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2026'
...