H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis Mondal, Moumita Ghorai, Pravat Samadder, Asmita Freunberger, Stefan Alexander ; https://orcid.org/0000-0003-2902-5319 Banerjee, Priyabrata Hydrogen peroxide (H2O2) is a crucial member of the reactive oxygen species (ROS) family, playing roles in cellular signalling and immune responses in human health. Moreover, it is a potential biomarker of diabetes when present in aberrant concentrations. Therefore, monitoring trace levels of H2O2 has become a research hotspot for analytical and sensor chemists. In this context, we report a rhodamine-based fluorescent probe (RN), which shows excellent fluorescent enhancement at 555 nm upon the addition of H2O2 along with a low limit of detection (LOD) of 0.67 ppm and fast response (∼2 min). The probe is highly selective for H2O2, showing no fluorescence enhancement with other ROS. RN is synthesised in a one-pot chemical reaction using rhodamine 6G (R6G) and 4,7,10-trioxa-1,13-tridecanediamine (TTDA). H2O2 detection in pre-treated milk samples proves its real-world viability. We found that RN shows low cytotoxicity, which allowed us to successfully explore its potential to monitor H2O2 generation in a diabetic L929 skin cell line and diabetic mice liver tissue. This result demonstrates promising features for assessing early diabetic progression through fluorescence imaging. Royal Society of Chemistry 2026 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/21730 Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. <i>Journal of Materials Chemistry B</i>. 2026. doi:<a href="https://doi.org/10.1039/d5tb02687c">10.1039/d5tb02687c</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1039/d5tb02687c info:eu-repo/semantics/altIdentifier/issn/2050-750X info:eu-repo/semantics/altIdentifier/e-issn/2050-7518 info:eu-repo/semantics/altIdentifier/pmid/41958432 info:eu-repo/semantics/closedAccess