article epub_ahead yes Moumita Mondal author Pravat Ghorai author Asmita Samadder author Stefan Alexander Freunberger author A8CA28E6-CE23-11E9-AD2D-EC27E66974250000-0003-2902-5319 Priyabrata Banerjee author StFr department Hydrogen peroxide (H2O2) is a crucial member of the reactive oxygen species (ROS) family, playing roles in cellular signalling and immune responses in human health. Moreover, it is a potential biomarker of diabetes when present in aberrant concentrations. Therefore, monitoring trace levels of H2O2 has become a research hotspot for analytical and sensor chemists. In this context, we report a rhodamine-based fluorescent probe (RN), which shows excellent fluorescent enhancement at 555 nm upon the addition of H2O2 along with a low limit of detection (LOD) of 0.67 ppm and fast response (∼2 min). The probe is highly selective for H2O2, showing no fluorescence enhancement with other ROS. RN is synthesised in a one-pot chemical reaction using rhodamine 6G (R6G) and 4,7,10-trioxa-1,13-tridecanediamine (TTDA). H2O2 detection in pre-treated milk samples proves its real-world viability. We found that RN shows low cytotoxicity, which allowed us to successfully explore its potential to monitor H2O2 generation in a diabetic L929 skin cell line and diabetic mice liver tissue. This result demonstrates promising features for assessing early diabetic progression through fluorescence imaging. Royal Society of Chemistry2026 eng 2050-750X 2050-7518 4195843210.1039/d5tb02687c Mondal, Moumita, Pravat Ghorai, Asmita Samadder, Stefan Alexander Freunberger, and Priyabrata Banerjee. “H2O2 Responsive Rhodamine-Based Probe for Monitoring Early-Stage Diabetes Diagnosis.” <i>Journal of Materials Chemistry B</i>. Royal Society of Chemistry, 2026. <a href="https://doi.org/10.1039/d5tb02687c">https://doi.org/10.1039/d5tb02687c</a>. Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. <i>Journal of Materials Chemistry B</i>. 2026. doi:<a href="https://doi.org/10.1039/d5tb02687c">10.1039/d5tb02687c</a> Mondal, M., Ghorai, P., Samadder, A., Freunberger, S. A., &#38; Banerjee, P. (2026). H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. <i>Journal of Materials Chemistry B</i>. Royal Society of Chemistry. <a href="https://doi.org/10.1039/d5tb02687c">https://doi.org/10.1039/d5tb02687c</a> Mondal, Moumita, et al. “H2O2 Responsive Rhodamine-Based Probe for Monitoring Early-Stage Diabetes Diagnosis.” <i>Journal of Materials Chemistry B</i>, Royal Society of Chemistry, 2026, doi:<a href="https://doi.org/10.1039/d5tb02687c">10.1039/d5tb02687c</a>. Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. 2026. H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. Journal of Materials Chemistry B. M. Mondal, P. Ghorai, A. Samadder, S. A. Freunberger, and P. Banerjee, “H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis,” <i>Journal of Materials Chemistry B</i>. Royal Society of Chemistry, 2026. M. Mondal, P. Ghorai, A. Samadder, S.A. Freunberger, P. Banerjee, Journal of Materials Chemistry B (2026). 217302026-04-13T07:45:26Z2026-04-16T05:44:49Z