[{"date_created":"2026-04-16T13:51:29Z","article_type":"original","year":"2026","DOAJ_listed":"1","file":[{"date_created":"2026-05-04T11:58:51Z","success":1,"file_id":"21793","checksum":"82098dd9d0ca609119f9f2c6beb4fc1e","creator":"dernst","file_name":"2026_CellReports_Li.pdf","access_level":"open_access","file_size":38532865,"content_type":"application/pdf","relation":"main_file","date_updated":"2026-05-04T11:58:51Z"}],"doi":"10.1016/j.celrep.2025.116904","publication_status":"published","oa":1,"intvolume":"        45","file_date_updated":"2026-05-04T11:58:51Z","author":[{"full_name":"Li, Yuxi","last_name":"Li","first_name":"Yuxi"},{"first_name":"Trevor C.","last_name":"Butler","full_name":"Butler, Trevor C."},{"full_name":"Nardone, Stefano","first_name":"Stefano","last_name":"Nardone"},{"first_name":"Christopher L.","last_name":"Jacobs","full_name":"Jacobs, Christopher L."},{"id":"de5f6fda-80fb-11ef-996f-a8c4ecd8e289","full_name":"Douglass, Amelia May Barnett","first_name":"Amelia May Barnett","last_name":"Douglass","orcid":"0000-0001-5398-6473"},{"last_name":"Madara","first_name":"Joseph C.","full_name":"Madara, Joseph C."},{"full_name":"McDonough, Miriam C.","last_name":"McDonough","first_name":"Miriam C."},{"first_name":"Jenkang","last_name":"Tao","full_name":"Tao, Jenkang"},{"first_name":"Elijah D.","last_name":"Lowenstein","full_name":"Lowenstein, Elijah D."},{"full_name":"Wang, Luhong","last_name":"Wang","first_name":"Luhong"},{"full_name":"Pant, Deepti","last_name":"Pant","first_name":"Deepti"},{"full_name":"Walker, Samuel J.","first_name":"Samuel J.","last_name":"Walker"},{"first_name":"Annette","last_name":"Wang","full_name":"Wang, Annette"},{"full_name":"Srinivasan, Harini","first_name":"Harini","last_name":"Srinivasan"},{"full_name":"Yang, Zongfang","last_name":"Yang","first_name":"Zongfang"},{"full_name":"Campbell, John N.","first_name":"John N.","last_name":"Campbell"},{"last_name":"Tsai","first_name":"Linus T.","full_name":"Tsai, Linus T."},{"last_name":"Lowell","first_name":"Bradford B.","full_name":"Lowell, Bradford B."},{"last_name":"Resch","first_name":"Jon M.","full_name":"Resch, Jon M."}],"language":[{"iso":"eng"}],"ddc":["570"],"has_accepted_license":"1","tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"department":[{"_id":"AmDo"}],"issue":"2","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21744","pmid":1,"OA_place":"publisher","publication":"Cell Reports","day":"24","title":"A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types","acknowledgement":"We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford\r\nSaper, as well as the Lowell, Tsai, and Resch laboratories for helpful discussions;\r\nAlysia Berns, Jia Yu, and Yanfang Li for technical support; the BNORC\r\nFunctional Genomics and Bioinformatics Core (P30DK046200) and the Iowa\r\nInstitute for Human Genetics Genomics Division (IIHG, RRID: SCR_023422)\r\nfor helpful discussions and technical assistance with sc/snRNA-seq; Zachary\r\nNiziolek and the Bauer Core Facility at Harvard University, the BIDMC Flow Cytometry\r\nCore, and Heath Vignes, Michael Shey, and Thomas Kaufman of the\r\nFlow Cytometry Facility at the University of Iowa Carver College of Medicine\r\nfor helpful discussions and technical support; the ICCB-Longwood Screening\r\nFacility of Harvard Medical School for assistance with the snRNA-seq\r\nexperiments; Dr. Sayak Mitter and Vizgen support for technical assistance\r\nwith the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin\r\nfor their assistance with MERSCOPE experiments within the Iowa\r\nNeuroBank Core in the Iowa Neuroscience Institute at the University of Iowa\r\nCarver College of Medicine. This research was funded by the following NIH\r\ngrants to L.T.T.: R01DK128406; to B.B.L.: R01DK075632, R01DK134427,\r\nand R01DK096010; to J.M.R.: R00HL144923 and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by a pharmacological\r\nsciences predoctoral training grant T32GM144636. Additional funding\r\nto J.M.R. came from the American Heart Association (AHA 935362), a University\r\nof Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and\r\nFeasibility Catalyst Grant, and an Iowa Neuroscience Institute Early Stage\r\nInvestigator award from the Carver Trust. Y.L. was supported by a predoctoral\r\nfellowship from the American Heart Association (AHA 25PRE1372983). A.M.D.\r\nwas supported by a postdoctoral fellowship from the Charles A. King Trust.","date_published":"2026-02-24T00:00:00Z","article_processing_charge":"Yes","volume":45,"month":"02","external_id":{"pmid":["41581146"]},"quality_controlled":"1","date_updated":"2026-05-04T12:00:31Z","publisher":"Elsevier","oa_version":"Published Version","scopus_import":"1","article_number":"116904","citation":{"apa":"Li, Y., Butler, T. C., Nardone, S., Jacobs, C. L., Douglass, A. M., Madara, J. C., … Resch, J. M. (2026). A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>","chicago":"Li, Yuxi, Trevor C. Butler, Stefano Nardone, Christopher L. Jacobs, Amelia M. Douglass, Joseph C. Madara, Miriam C. McDonough, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>.","mla":"Li, Yuxi, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>, vol. 45, no. 2, 116904, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>.","short":"Y. Li, T.C. Butler, S. Nardone, C.L. Jacobs, A.M. Douglass, J.C. Madara, M.C. McDonough, J. Tao, E.D. Lowenstein, L. Wang, D. Pant, S.J. Walker, A. Wang, H. Srinivasan, Z. Yang, J.N. Campbell, L.T. Tsai, B.B. Lowell, J.M. Resch, Cell Reports 45 (2026).","ista":"Li Y, Butler TC, Nardone S, Jacobs CL, Douglass AM, Madara JC, McDonough MC, Tao J, Lowenstein ED, Wang L, Pant D, Walker SJ, Wang A, Srinivasan H, Yang Z, Campbell JN, Tsai LT, Lowell BB, Resch JM. 2026. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. Cell Reports. 45(2), 116904.","ieee":"Y. Li <i>et al.</i>, “A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types,” <i>Cell Reports</i>, vol. 45, no. 2. Elsevier, 2026.","ama":"Li Y, Butler TC, Nardone S, et al. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. 2026;45(2). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>"},"abstract":[{"text":"The paraventricular hypothalamus (PVH) controls behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for centrally projecting PVH neuron populations remain largely undefined, and a complete census of PVH cell types has not been established. Therefore, we performed extensive single-cell/nucleus RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding areas. Additionally, projection-based profiling identified neurons that project to the parabrachial region (PB) and spinal cord, helping to determine PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and silencing them causes obesity. Together, this atlas contributes high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis.","lang":"eng"}],"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"type":"journal_article","status":"public","OA_type":"gold"}]