---
OA_place: repository
OA_type: green
_id: '21848'
abstract:
- lang: eng
  text: 'Despite the success of mRNA therapeutics, challenges remain in optimizing
    immune responses and minimizing side effects. Cell-specific antigen delivery may
    help reduce required doses and improve vaccine efficacy. In this study, we report
    on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting
    cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific
    glycomimetic ligand, we achieve selective mRNA delivery to both murine and human
    primary Langerhans cells with minimal off-target uptake, at the same time resulting
    in significantly increased mRNA translation. This targeted mRNA delivery not only
    enhances antigen presentation and T-cell responses but also enables dose-sparing
    and superior antitumor immunity compared with conventional immunization in a B16-OVA
    tumor model. Importantly, our platform’s high compatibility with various lipid
    nanoparticle formulations offers a flexible and precise tool for skin-directed
    mRNA delivery.'
acknowledged_ssus:
- _id: PreCl
acknowledgement: We thank Mareike Rentzsch for her intellectual contributions during
  the course of our discussions. We thank Michael Schunn from the Preclinical Facility
  of the Institute of Science and Technology Austria for his continuous technical
  support. Guarantor of the work is FS. This project was supported by “Seedfinancing”
  (P2282679) of the Austrian Federal Ministry of Digital and Economic Affairs and
  the Ministry of Climate Action and Energy, handled by the Austrian Wirtschaftsservice,
  as well as by...
article_processing_charge: No
article_type: original
author:
- first_name: Klara
  full_name: Klein, Klara
  last_name: Klein
- first_name: Litty
  full_name: Johnson, Litty
  last_name: Johnson
- first_name: Ramona
  full_name: Rîca, Ramona
  last_name: Rîca
- first_name: Mirza
  full_name: Sarcevic, Mirza
  last_name: Sarcevic
- first_name: Gabriele
  full_name: Carta, Gabriele
  last_name: Carta
- first_name: Saskia
  full_name: Seiser, Saskia
  last_name: Seiser
- first_name: Adelheid
  full_name: Elbe-Bürger, Adelheid
  last_name: Elbe-Bürger
- first_name: Freyja
  full_name: Langer, Freyja
  id: 3C1BE782-F248-11E8-B48F-1D18A9856A87
  last_name: Langer
- first_name: Nowras
  full_name: Rahhal, Nowras
  last_name: Rahhal
- first_name: Christoph
  full_name: Rademacher, Christoph
  last_name: Rademacher
- first_name: Robert
  full_name: Wawrzinek, Robert
  last_name: Wawrzinek
- first_name: Federica
  full_name: Quattrone, Federica
  last_name: Quattrone
- first_name: Florian
  full_name: Sparber, Florian
  last_name: Sparber
citation:
  ama: 'Klein K, Johnson L, Rîca R, et al. Langerhans cell–targeted mRNA delivery:
    A strategy for dose-sparing and enhanced antitumor immunity. <i>Journal of Investigative
    Dermatology</i>. doi:<a href="https://doi.org/10.1016/j.jid.2026.03.026">10.1016/j.jid.2026.03.026</a>'
  apa: 'Klein, K., Johnson, L., Rîca, R., Sarcevic, M., Carta, G., Seiser, S., … Sparber,
    F. (n.d.). Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing
    and enhanced antitumor immunity. <i>Journal of Investigative Dermatology</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.jid.2026.03.026">https://doi.org/10.1016/j.jid.2026.03.026</a>'
  chicago: 'Klein, Klara, Litty Johnson, Ramona Rîca, Mirza Sarcevic, Gabriele Carta,
    Saskia Seiser, Adelheid Elbe-Bürger, et al. “Langerhans Cell–Targeted MRNA Delivery:
    A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative
    Dermatology</i>. Elsevier, n.d. <a href="https://doi.org/10.1016/j.jid.2026.03.026">https://doi.org/10.1016/j.jid.2026.03.026</a>.'
  ieee: 'K. Klein <i>et al.</i>, “Langerhans cell–targeted mRNA delivery: A strategy
    for dose-sparing and enhanced antitumor immunity,” <i>Journal of Investigative
    Dermatology</i>. Elsevier.'
  ista: 'Klein K, Johnson L, Rîca R, Sarcevic M, Carta G, Seiser S, Elbe-Bürger A,
    Langer F, Rahhal N, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans
    cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor
    immunity. Journal of Investigative Dermatology.'
  mla: 'Klein, Klara, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for
    Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative Dermatology</i>,
    Elsevier, doi:<a href="https://doi.org/10.1016/j.jid.2026.03.026">10.1016/j.jid.2026.03.026</a>.'
  short: K. Klein, L. Johnson, R. Rîca, M. Sarcevic, G. Carta, S. Seiser, A. Elbe-Bürger,
    F. Langer, N. Rahhal, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Journal
    of Investigative Dermatology (n.d.).
date_created: 2026-05-10T22:02:16Z
date_published: 2026-04-07T00:00:00Z
date_updated: 2026-05-11T06:07:32Z
day: '07'
department:
- _id: PreCl
doi: 10.1016/j.jid.2026.03.026
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2025.06.25.661517
month: '04'
oa: 1
oa_version: Preprint
publication: Journal of Investigative Dermatology
publication_identifier:
  eissn:
  - 1523-1747
  issn:
  - 0022-202X
publication_status: inpress
publisher: Elsevier
scopus_import: '1'
status: public
title: 'Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced
  antitumor immunity'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...