[{"oa_version":"Published Version","title":"α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures","type":"journal_article","pmid":1,"scopus_import":"1","day":"27","publication":"Journal of Cell Science","external_id":{"pmid":["41834724"]},"file":[{"date_updated":"2026-05-12T06:27:54Z","content_type":"application/pdf","file_name":"2026_JourCellScience_Goeschl.pdf","date_created":"2026-05-12T06:27:54Z","access_level":"open_access","file_size":1957057,"checksum":"8db35c97588c2f6ef88c7e8d5924cf8c","relation":"main_file","file_id":"21861","success":1,"creator":"dernst"}],"citation":{"ista":"Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H. 2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. 139(8), jcs264420.","mla":"Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>, vol. 139, no. 8, jcs264420, The Company of Biologists, 2026, doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>.","ama":"Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. 2026;139(8). doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>","chicago":"Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>. The Company of Biologists, 2026. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>.","ieee":"V. Goeschl <i>et al.</i>, “α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures,” <i>Journal of Cell Science</i>, vol. 139, no. 8. The Company of Biologists, 2026.","apa":"Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V., &#38; Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>","short":"V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H. Kubista, Journal of Cell Science 139 (2026)."},"article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","doi":"10.1242/jcs.264420","article_number":"jcs264420","_id":"21860","date_updated":"2026-05-12T06:40:18Z","publication_status":"published","month":"04","intvolume":"       139","quality_controlled":"1","date_published":"2026-04-27T00:00:00Z","department":[{"_id":"Bio"}],"PlanS_conform":"1","author":[{"first_name":"Vanessa","last_name":"Goeschl","full_name":"Goeschl, Vanessa"},{"full_name":"Hotka, Matej","last_name":"Hotka","first_name":"Matej"},{"last_name":"Hochreiter","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","first_name":"Bernhard","full_name":"Hochreiter, Bernhard"},{"full_name":"Hilber, Karlheinz","first_name":"Karlheinz","last_name":"Hilber"},{"last_name":"Boehm","first_name":"Stefan","full_name":"Boehm, Stefan"},{"first_name":"Andrey V.","last_name":"Kozlov","full_name":"Kozlov, Andrey V."},{"last_name":"Kubista","first_name":"Helmut","full_name":"Kubista, Helmut"}],"year":"2026","language":[{"iso":"eng"}],"ddc":["570"],"status":"public","file_date_updated":"2026-05-12T06:27:54Z","date_created":"2026-05-11T10:52:27Z","oa":1,"publisher":"The Company of Biologists","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","OA_place":"publisher","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"volume":139,"issue":"8","acknowledgement":"The technical assistance by Tanja Wagner and Elena Lilliu is gratefully acknowledged. This research was funded in whole or in part by the Austrian Science Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access funding provided by Medical University of Vienna and the Austrian Science Fund (FWF). Deposited in PMC for immediate release.","abstract":[{"lang":"eng","text":"Glutamate excitotoxicity is a cell death mechanism triggered by accumulation of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex (αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point controlling glutamate formation and its consumption as a fuel. Hence, modulation of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic effects. To address this hypothesis, hippocampal neurons in primary co-culture with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover, NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA receptors involved mGluR1 activation and was accompanied by enhanced neuronal discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses evoked by glutamate application. These results suggest that mGluR1-mediated potentiation of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might exacerbate glutamate excitotoxicity."}]}]