---
res:
  bibo_abstract:
  - A hallmark of immune cell trafficking is directional guidance via gradients of
    soluble or surface bound chemokines. Vascular endothelial cells produce, transport
    and deposit either their own chemokines or chemokines produced by the underlying
    stroma. Endothelial heparan sulfate (HS) was suggested to be a critical scaffold
    for these chemokine pools, but it is unclear how steep chemokine gradients are
    sustained between the lumenal and ablumenal aspects of blood vessels. Addressing
    this question by semi-quantitative immunostaining of HS moieties around blood
    vessels with a pan anti-HS IgM mAb, we found a striking HS enrichment in the basal
    lamina of resting and inflamed post capillary skin venules, as well as in high
    endothelial venules (HEVs) of lymph nodes. Staining of skin vessels with a glycocalyx
    probe further suggested that their lumenal glycocalyx contains much lower HS density
    than their basolateral extracellular matrix (ECM). This polarized HS pattern was
    observed also in isolated resting and inflamed microvascular dermal cells. Notably,
    progressive skin inflammation resulted in massive ECM deposition and in further
    HS enrichment around skin post capillary venules and their associated pericytes.
    Inflammation-dependent HS enrichment was not compromised in mice deficient in
    the main HS degrading enzyme, heparanase. Our results suggest that the blood vasculature
    patterns steep gradients of HS scaffolds between their lumenal and basolateral
    endothelial aspects, and that inflammatory processes can further enrich the HS
    content nearby inflamed vessels. We propose that chemokine gradients between the
    lumenal and ablumenal sides of vessels could be favored by these sharp HS scaffold
    gradients.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Liat
      foaf_name: Stoler Barak, Liat
      foaf_surname: Stoler Barak
  - foaf_Person:
      foaf_givenName: Christine
      foaf_name: Moussion, Christine
      foaf_surname: Moussion
      foaf_workInfoHomepage: http://www.librecat.org/personId=3356F664-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Elias
      foaf_name: Shezen, Elias
      foaf_surname: Shezen
  - foaf_Person:
      foaf_givenName: Miki
      foaf_name: Hatzav, Miki
      foaf_surname: Hatzav
  - foaf_Person:
      foaf_givenName: Michael K
      foaf_name: Sixt, Michael K
      foaf_surname: Sixt
      foaf_workInfoHomepage: http://www.librecat.org/personId=41E9FBEA-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-6620-9179
  - foaf_Person:
      foaf_givenName: Ronen
      foaf_name: Alon, Ronen
      foaf_surname: Alon
  bibo_doi: 10.1371/journal.pone.0085699
  bibo_issue: '1'
  bibo_volume: 9
  dct_date: 2014^xs_gYear
  dct_identifier:
  - UT:000330283100061
  dct_language: eng
  dct_publisher: Public Library of Science@
  dct_title: Blood vessels pattern heparan sulfate gradients between their apical
    and basolateral aspects@
...