{"department":[{"_id":"PeJo"}],"publication_identifier":{"issn":["0036-8075"]},"intvolume":"       343","volume":343,"project":[{"grant_number":"P24909-B24","_id":"25C26B1E-B435-11E9-9278-68D0E5697425","name":"Mechanisms of transmitter release at GABAergic synapses","call_identifier":"FWF"},{"grant_number":"268548","call_identifier":"FP7","_id":"25C0F108-B435-11E9-9278-68D0E5697425","name":"Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons"}],"date_updated":"2025-04-15T07:20:00Z","quality_controlled":"1","publication_status":"published","doi":"10.1126/science.1244811","ec_funded":1,"author":[{"id":"36C4978E-F248-11E8-B48F-1D18A9856A87","last_name":"Vyleta","full_name":"Vyleta, Nicholas","first_name":"Nicholas"},{"first_name":"Peter M","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","last_name":"Jonas"}],"page":"665 - 670","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617475/"}],"language":[{"iso":"eng"}],"status":"public","_id":"2229","abstract":[{"lang":"eng","text":"The distance between Ca^2+ channels and release sensors determines the speed and efficacy of synaptic transmission. Tight "nanodomain" channel-sensor coupling initiates transmitter release at synapses in the mature brain, whereas loose "microdomain" coupling appears restricted to early developmental stages. To probe the coupling configuration at a plastic synapse in the mature central nervous system, we performed paired recordings between mossy fiber terminals and CA3 pyramidal neurons in rat hippocampus. Millimolar concentrations of both the fast Ca^2+ chelator BAPTA [1,2-bis(2-aminophenoxy)ethane- N,N, N′,N′-tetraacetic acid] and the slow chelator EGTA efficiently suppressed transmitter release, indicating loose coupling between Ca^2+ channels and release sensors. Loose coupling enabled the control of initial release probability by fast endogenous Ca^2+ buffers and the generation of facilitation by buffer saturation. Thus, loose coupling provides the molecular framework for presynaptic plasticity."}],"publist_id":"4732","date_created":"2018-12-11T11:56:27Z","date_published":"2014-02-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Science","month":"02","year":"2014","title":"Loose coupling between Ca^2+ channels and release sensors at a plastic hippocampal synapse","oa_version":"Submitted Version","type":"journal_article","issue":"6171","publisher":"American Association for the Advancement of Science","day":"01","article_processing_charge":"No","corr_author":"1","scopus_import":"1","oa":1}