[{"oa":1,"month":"05","author":[{"first_name":"Jonathan G.G.","last_name":"Kaufman","full_name":"Kaufman, Jonathan G.G."},{"last_name":"Tagiltsev","first_name":"Grigory","full_name":"Tagiltsev, Grigory"},{"first_name":"Danièle S.","last_name":"Stalder","full_name":"Stalder, Danièle S."},{"last_name":"Taylor","first_name":"Rebecca J.","full_name":"Taylor, Rebecca J."},{"full_name":"Sava, Ioana","first_name":"Ioana","last_name":"Sava"},{"first_name":"Hui","last_name":"Guo","full_name":"Guo, Hui"},{"first_name":"Katarzyna A.","last_name":"Ciazynska","full_name":"Ciazynska, Katarzyna A."},{"full_name":"Zaccai, Nathan R.","last_name":"Zaccai","first_name":"Nathan R."},{"last_name":"Gray","first_name":"Sally R.","full_name":"Gray, Sally R."},{"last_name":"Vallis","first_name":"Yvonne","id":"05A2795C-31B5-11EA-83A7-7DA23DDC885E","orcid":"0000-0002-5408-5906","full_name":"Vallis, Yvonne"},{"full_name":"Höning, Stefan","last_name":"Höning","first_name":"Stefan"},{"full_name":"Kelly, Bernard T.","last_name":"Kelly","first_name":"Bernard T."},{"full_name":"Gershlick, David C.","last_name":"Gershlick","first_name":"David C."},{"full_name":"Briggs, John A.G.","first_name":"John A.G.","last_name":"Briggs"},{"full_name":"Owen, David J.","last_name":"Owen","first_name":"David J."}],"dataavailabilitystatement":"Structures determined by electron microscopy are deposited in the Electron\r\nMicroscopy Data Bank under accession codes EMD-54255,\r\nEMD-54256,\r\nEMD-54257,\r\nand\r\nEMD-54258.\r\nCorresponding molecular models are deposited in the Protein Data Bank under\r\naccession codes 9RTW, 9RTX, 9RTY, and 9RTZ. All additional data and code required to\r\nevaluate and reproduce the results in the paper are present in the paper and/or the\r\nsupplementary materials. Any code used is explicitly stated and available in the original\r\npublications. Plasmids and cell lines can be requested by writing to D.C.G. (dg553@ cam. ac. uk)\r\nor D.J.O. (djo30@ cam. ac. uk). All reasonable requests for materials will be honored.","quality_controlled":"1","volume":12,"date_created":"2026-07-13T10:52:15Z","year":"2026","article_number":"eaed1529","day":"15","publication_identifier":{"eissn":["2375-2548"]},"OA_place":"publisher","file_date_updated":"2026-07-14T06:45:37Z","acknowledgement":"We thank P. Luzio, N. Bright, and M. S. Robinson for helpful discussions;\r\nJ. Stacey and Y. Fujiharu for technical discussions and assistance; F. Beck for assistance with\r\ncomputing infrastructure; and M. Riggi for discussion of data presentation and figure design.\r\nCryo-E\r\nM data was collected at the Department of Cell and Virus Structure, Max Planck Institute\r\nof Biochemistry. Funding: This work was funded by: the Wellcome Trust, Wellcome Discovery\r\nAward 227915/Z/23/Z (to D.J.O., J.A.G.B., and D.G.S.) and Wellcome PRF 207455/Z/17/Z (to\r\nD.J.O.); the Max Planck Society (to J.A.G.B.); the Medical Research Council (UKRI) MC_\r\nUP_1201/16 (to J.A.G.B.) and MRC DT P MR/N013433/1 (to J.G.G.K.); EMBO Long-term\r\nPostdoctoral Fellowship ALT F-383-\r\n2022\r\n(to G.T.); and the Wellcome Trust/Royal Society, Sir\r\nHenry Dale Fellowship 210481 (to D.C.G) and the Biotechnology and Biological Sciences\r\nResearch Council (UKRI) responsive mode grants BB/W005905/1 and UKRI715 (to D.C.G.).","date_published":"2026-05-15T00:00:00Z","supplementarymaterial":"yes","has_accepted_license":"1","das_tickbox":"1","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"publisher":"American Association for the Advancement of Science","citation":{"ista":"Kaufman JGG, Tagiltsev G, Stalder DS, Taylor RJ, Sava I, Guo H, Ciazynska KA, Zaccai NR, Gray SR, Vallis Y, Höning S, Kelly BT, Gershlick DC, Briggs JAG, Owen DJ. 2026. Architecture of clathrin-independent AP3:ARF1-coated carriers. Science Advances. 12(20), eaed1529.","ama":"Kaufman JGG, Tagiltsev G, Stalder DS, et al. Architecture of clathrin-independent AP3:ARF1-coated carriers. <i>Science Advances</i>. 2026;12(20). doi:<a href=\"https://doi.org/10.1126/sciadv.aed1529\">10.1126/sciadv.aed1529</a>","mla":"Kaufman, Jonathan G. G., et al. “Architecture of Clathrin-Independent AP3:ARF1-Coated Carriers.” <i>Science Advances</i>, vol. 12, no. 20, eaed1529, American Association for the Advancement of Science, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aed1529\">10.1126/sciadv.aed1529</a>.","short":"J.G.G. Kaufman, G. Tagiltsev, D.S. Stalder, R.J. Taylor, I. Sava, H. Guo, K.A. Ciazynska, N.R. Zaccai, S.R. Gray, Y. Vallis, S. Höning, B.T. Kelly, D.C. Gershlick, J.A.G. Briggs, D.J. Owen, Science Advances 12 (2026).","chicago":"Kaufman, Jonathan G.G., Grigory Tagiltsev, Danièle S. Stalder, Rebecca J. Taylor, Ioana Sava, Hui Guo, Katarzyna A. Ciazynska, et al. “Architecture of Clathrin-Independent AP3:ARF1-Coated Carriers.” <i>Science Advances</i>. American Association for the Advancement of Science, 2026. <a href=\"https://doi.org/10.1126/sciadv.aed1529\">https://doi.org/10.1126/sciadv.aed1529</a>.","apa":"Kaufman, J. G. G., Tagiltsev, G., Stalder, D. S., Taylor, R. J., Sava, I., Guo, H., … Owen, D. J. (2026). Architecture of clathrin-independent AP3:ARF1-coated carriers. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aed1529\">https://doi.org/10.1126/sciadv.aed1529</a>","ieee":"J. G. G. Kaufman <i>et al.</i>, “Architecture of clathrin-independent AP3:ARF1-coated carriers,” <i>Science Advances</i>, vol. 12, no. 20. American Association for the Advancement of Science, 2026."},"abstract":[{"lang":"eng","text":"The AP3 complex mediates cargo sorting and carrier assembly for the trafficking of transmembrane proteins from endosomes to lysosomes. AP3 is generally believed to localize to clathrin-free, ARF1-positive, elongated carriers in cells, but the architecture of AP3-based coats was unknown. Using in vitro reconstitution and cryo–electron tomography, we demonstrate that AP3:ARF1 spontaneously remodels membranes containing cargo and the phosphoinositide PI(3,5)P\r\n                    <jats:sub>2</jats:sub>\r\n                    into tubular structures coated in spiraling rows of AP3 arches and ARF1 dimers. Targeted point mutations disrupting critical AP3:ARF1 and AP3:AP3 lattice interfaces disrupt AP3 recruitment, carrier formation, and lysosomal cargo trafficking in cells. We propose that AP3 generates tubular carriers on endosomes by organizing ARF1 dimers into elongated membrane-deforming arrays while simultaneously selecting cargo. By demonstrating that AP3:ARF1 can generate carriers without using a clathrin lattice, we explain the clathrin independence of AP3-mediated trafficking."}],"publication":"Science Advances","researchdata_availability":"yes","DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Architecture of clathrin-independent AP3:ARF1-coated carriers","department":[{"_id":"MaDe"}],"PlanS_conform":"1","oa_version":"Published Version","type":"journal_article","article_type":"original","article_processing_charge":"Yes","OA_type":"gold","language":[{"iso":"eng"}],"_id":"22306","file":[{"file_id":"22331","creator":"dernst","relation":"main_file","file_size":8269526,"checksum":"461b5f73941fe6b5df2a129c7563601f","file_name":"2026_ScienceAdv_Kaufman.pdf","date_updated":"2026-07-14T06:45:37Z","access_level":"open_access","date_created":"2026-07-14T06:45:37Z","content_type":"application/pdf","success":1}],"ddc":["570"],"publication_status":"published","issue":"20","status":"public","date_updated":"2026-07-14T06:52:00Z","intvolume":"        12","scopus_import":"1","doi":"10.1126/sciadv.aed1529"}]