---
res:
  bibo_abstract:
  - RNA polymerase II (Pol II) must be assembled in the cytoplasm before it enters
    the nucleus, where it transcribes protein-coding genes. Although transcription
    by Pol II is intensively studied, how this central multi-subunit enzyme is made
    and the role of dedicated assembly factors remains unclear. Here, we report the
    integrative structural analysis of a native human Pol II from the cytoplasm captured
    near the end of biogenesis. The complex contains Gdown1 and three biogenesis factors
    – RPAP2 and the critical small GTPases GPN1 and GPN3. Cryo-EM analysis of the
    complex reveals how Gdown1 and RPAP2 associate with Pol II and prevent the premature
    association of transcription factors. Further biochemical and cryo-EM analysis
    reveals how RPAP2 tethers GPN1–GPN3 to the complex and how the assembly of the
    RPAP2–GPN1–GPN3 complex is controlled by GTP hydrolysis. The combined results
    uncover a network of interactions that chaperone cytoplasmic Pol II to prevent
    aberrant interactions, reveal a molecular switch regulating biogenesis factor
    association, and suggest a general mechanism for the action of GPN-loop GTPase
    family of enzymes.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Annamaria
      foaf_name: Hlavata, Annamaria
      foaf_surname: Hlavata
      foaf_workInfoHomepage: http://www.librecat.org/personId=36062FEC-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Benjamin
      foaf_name: Neuditschko, Benjamin
      foaf_surname: Neuditschko
  - foaf_Person:
      foaf_givenName: Ulla
      foaf_name: Schellhaas, Ulla
      foaf_surname: Schellhaas
  - foaf_Person:
      foaf_givenName: Clemens
      foaf_name: Plaschka, Clemens
      foaf_surname: Plaschka
  - foaf_Person:
      foaf_givenName: Franz
      foaf_name: Herzog, Franz
      foaf_surname: Herzog
  - foaf_Person:
      foaf_givenName: Carrie A
      foaf_name: Bernecky, Carrie A
      foaf_surname: Bernecky
      foaf_workInfoHomepage: http://www.librecat.org/personId=2CB9DFE2-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-0893-7036
  bibo_doi: 10.1038/s41467-026-75416-8
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2041-1723
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Structure of cytoplasmic RNA polymerase II@
...