---
res:
  bibo_abstract:
  - Phase plates can, in principle, overcome the poor image contrast in cryo–electron
    microscopy (cryo-EM) and the resulting limits on the structural reconstruction
    of small proteins. However, previous designs have been unstable and compromised
    the high-resolution signal and have thus been unable to surpass results achieved
    by standard cryo-EM. Here, we show that the laser phase plate (LPP), installed
    in a modern, custom Titan Krios microscope, enhances the resolution in single-particle
    reconstruction of small proteins by improving specimen-motion correction and recovery
    of information from the early frames, as well as particle visualization, three-dimensional
    classification, and alignment. These advances use standard defocus ranges and
    reconstruction procedures but open the door to LPP-tailored protocols, offering
    further improvements by leveraging the LPP demonstrated here.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Petar N
      foaf_name: Petrov, Petar N
      foaf_surname: Petrov
      foaf_workInfoHomepage: http://www.librecat.org/personId=b1d6732d-8cb6-11f0-baab-bd460ee3a287
  - foaf_Person:
      foaf_givenName: Jessie T.
      foaf_name: Zhang, Jessie T.
      foaf_surname: Zhang
  - foaf_Person:
      foaf_givenName: Jonathan
      foaf_name: Remis, Jonathan
      foaf_surname: Remis
  - foaf_Person:
      foaf_givenName: Jeremy J.
      foaf_name: Axelrod, Jeremy J.
      foaf_surname: Axelrod
  - foaf_Person:
      foaf_givenName: Hang
      foaf_name: Cheng, Hang
      foaf_surname: Cheng
  - foaf_Person:
      foaf_givenName: Eric S.
      foaf_name: Cooper, Eric S.
      foaf_surname: Cooper
  - foaf_Person:
      foaf_givenName: Ian K.
      foaf_name: Hicklin, Ian K.
      foaf_surname: Hicklin
  - foaf_Person:
      foaf_givenName: Shahar
      foaf_name: Sandhaus, Shahar
      foaf_surname: Sandhaus
  - foaf_Person:
      foaf_givenName: Cooper
      foaf_name: Schnurr, Cooper
      foaf_surname: Schnurr
  - foaf_Person:
      foaf_givenName: Robert M.
      foaf_name: Glaeser, Robert M.
      foaf_surname: Glaeser
  - foaf_Person:
      foaf_givenName: Holger
      foaf_name: Müller, Holger
      foaf_surname: Müller
  bibo_doi: 10.1126/science.aeh0665
  bibo_issue: '6807'
  bibo_volume: 393
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0036-8075
  - http://id.crossref.org/issn/1095-9203
  dct_language: eng
  dct_publisher: AAAS@
  dct_title: Laser phase plate improves structure determination of small proteins
    by cryo-EM@
  fabio_hasPubmedId: '42275466'
...