---
res:
  bibo_abstract:
  - Exogenous application of biologically important molecules for plant growth promotion
    and/or regulation is very common both in plant research and horticulture. Plant
    hormones such as auxins and cytokinins are classes of compounds which are often
    applied exogenously. Nevertheless, plants possess a well-established machinery
    to regulate the active pool of exogenously applied compounds by converting them
    to metabolites and conjugates. Consequently, it is often very useful to know the
    in vivo status of applied compounds to connect them with some of the regulatory
    events in plant developmental processes. The in vivo status of applied compounds
    can be measured by incubating plants with radiolabeled compounds, followed by
    extraction, purification, and HPLC metabolic profiling of plant extracts. Recently
    we have used this method to characterize the intracellularly localized PIN protein,
    PIN5. Here we explain the method in detail, with a focus on general application.
    @eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Sibu
      foaf_name: Simon, Sibu
      foaf_surname: Simon
      foaf_workInfoHomepage: http://www.librecat.org/personId=4542EF9A-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-1998-6741
  - foaf_Person:
      foaf_givenName: Petr
      foaf_name: Skůpa, Petr
      foaf_surname: Skůpa
  - foaf_Person:
      foaf_givenName: Petre
      foaf_name: Dobrev, Petre
      foaf_surname: Dobrev
  - foaf_Person:
      foaf_givenName: Jan
      foaf_name: Petrášek, Jan
      foaf_surname: Petrášek
  - foaf_Person:
      foaf_givenName: Eva
      foaf_name: Zažímalová, Eva
      foaf_surname: Zažímalová
  - foaf_Person:
      foaf_givenName: Jirí
      foaf_name: Friml, Jirí
      foaf_surname: Friml
      foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8302-7596
  bibo_doi: 10.1007/978-1-62703-592-7_23
  bibo_volume: 1056
  dct_date: 2014^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1064-3745
  dct_language: eng
  dct_publisher: Springer@
  dct_title: 'Analyzing the in vivo status of exogenously applied auxins: A HPLC-based
    method to characterize the intracellularly localized auxin transporters@'
...
