---
_id: '2245'
abstract:
- lang: eng
  text: 'Exogenous application of biologically important molecules for plant growth
    promotion and/or regulation is very common both in plant research and horticulture.
    Plant hormones such as auxins and cytokinins are classes of compounds which are
    often applied exogenously. Nevertheless, plants possess a well-established machinery
    to regulate the active pool of exogenously applied compounds by converting them
    to metabolites and conjugates. Consequently, it is often very useful to know the
    in vivo status of applied compounds to connect them with some of the regulatory
    events in plant developmental processes. The in vivo status of applied compounds
    can be measured by incubating plants with radiolabeled compounds, followed by
    extraction, purification, and HPLC metabolic profiling of plant extracts. Recently
    we have used this method to characterize the intracellularly localized PIN protein,
    PIN5. Here we explain the method in detail, with a focus on general application. '
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Sibu
  full_name: Simon, Sibu
  id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
  last_name: Simon
  orcid: 0000-0002-1998-6741
- first_name: Petr
  full_name: Skůpa, Petr
  last_name: Skůpa
- first_name: Petre
  full_name: Dobrev, Petre
  last_name: Dobrev
- first_name: Jan
  full_name: Petrášek, Jan
  last_name: Petrášek
- first_name: Eva
  full_name: Zažímalová, Eva
  last_name: Zažímalová
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: 'Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the
    in vivo status of exogenously applied auxins: A HPLC-based method to characterize
    the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds.
    <i>Plant Chemical Genomics</i>. Vol 1056. Methods in Molecular Biology. Springer;
    2014:255-264. doi:<a href="https://doi.org/10.1007/978-1-62703-592-7_23">10.1007/978-1-62703-592-7_23</a>'
  apa: 'Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., &#38; Friml,
    J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based
    method to characterize the intracellularly localized auxin transporters. In G.
    Hicks &#38; S. Robert (Eds.), <i>Plant Chemical Genomics</i> (Vol. 1056, pp. 255–264).
    Springer. <a href="https://doi.org/10.1007/978-1-62703-592-7_23">https://doi.org/10.1007/978-1-62703-592-7_23</a>'
  chicago: 'Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and
    Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based
    Method to Characterize the Intracellularly Localized Auxin Transporters.” In <i>Plant
    Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64.
    Methods in Molecular Biology. Springer, 2014. <a href="https://doi.org/10.1007/978-1-62703-592-7_23">https://doi.org/10.1007/978-1-62703-592-7_23</a>.'
  ieee: 'S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml,
    “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method
    to characterize the intracellularly localized auxin transporters,” in <i>Plant
    Chemical Genomics</i>, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014,
    pp. 255–264.'
  ista: 'Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing
    the in vivo status of exogenously applied auxins: A HPLC-based method to characterize
    the intracellularly localized auxin transporters. In: Plant Chemical Genomics.
    Methods in Molecular Biology, vol. 1056, 255–264.'
  mla: 'Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins:
    A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.”
    <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, vol.
    1056, Springer, 2014, pp. 255–64, doi:<a href="https://doi.org/10.1007/978-1-62703-592-7_23">10.1007/978-1-62703-592-7_23</a>.'
  short: S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:,
    G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.
date_created: 2018-12-11T11:56:32Z
date_published: 2014-01-01T00:00:00Z
date_updated: 2025-07-10T11:52:16Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-1-62703-592-7_23
editor:
- first_name: Glenn
  full_name: Hicks, Glenn
  last_name: Hicks
- first_name: Stéphanie
  full_name: Robert, Stéphanie
  last_name: Robert
intvolume: '      1056'
language:
- iso: eng
month: '01'
oa_version: None
page: 255 - 264
publication: Plant Chemical Genomics
publication_identifier:
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
publist_id: '4704'
quality_controlled: '1'
scopus_import: '1'
series_title: Methods in Molecular Biology
status: public
title: 'Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method
  to characterize the intracellularly localized auxin transporters'
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1056
year: '2014'
...