---
res:
  bibo_abstract:
  - 'Mosaic Analysis with Double Markers (MADM) is a method for generating genetically
    mosaic mice, in which sibling mutant and wild-type cells are labeled with different
    fluorescent markers. It is a powerful tool that enables analysis of gene function
    at the single cell level in vivo. It requires transgenic cassettes to be located
    between the centromere and the mutation in the gene of interest on the same chromosome.
    Here we compare procedures for introduction of MADM cassettes into new loci in
    the mouse genome, and describe new approaches for expanding the utility of MADM.
    We show that: 1) Targeted homologous recombination outperforms random transgenesis
    in generation of reliably expressed MADM cassettes, 2) MADM cassettes in new genomic
    loci need to be validated for biallelic and ubiquitous expression, 3) Recombination
    between MADM cassettes on different chromosomes can be used to study reciprocal
    chromosomal deletions/duplications, and 4) MADM can be modified to permit transgene
    expression by combining it with a binary expression system. The advances described
    in this study expand current, and enable new and more versatile applications of
    MADM.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Bosiljka
      foaf_name: Tasic, Bosiljka
      foaf_surname: Tasic
  - foaf_Person:
      foaf_givenName: Kazunari
      foaf_name: Miyamichi, Kazunari
      foaf_surname: Miyamichi
  - foaf_Person:
      foaf_givenName: Simon
      foaf_name: Simon Hippenmeyer
      foaf_surname: Hippenmeyer
      foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-2279-1061
  - foaf_Person:
      foaf_givenName: Vardhan
      foaf_name: Dani, Vardhan S.
      foaf_surname: Dani
  - foaf_Person:
      foaf_givenName: H.
      foaf_name: Zeng, H.
      foaf_surname: Zeng
  - foaf_Person:
      foaf_givenName: William
      foaf_name: Joo, William
      foaf_surname: Joo
  - foaf_Person:
      foaf_givenName: Hui
      foaf_name: Zong, Hui
      foaf_surname: Zong
  - foaf_Person:
      foaf_givenName: Yanru
      foaf_name: Chen-Tsai, Yanru
      foaf_surname: Chen Tsai
  - foaf_Person:
      foaf_givenName: Liqun
      foaf_name: Luo, Liqun
      foaf_surname: Luo
  bibo_doi: 10.1371/journal.pone.0033332
  bibo_issue: '3'
  bibo_volume: 7
  dct_date: 2012^xs_gYear
  dct_publisher: Public Library of Science@
  dct_title: Extensions of MADM (Mosaic Analysis with Double Markers) in Mice @
...