---
_id: '2262'
abstract:
- lang: eng
  text: 'Mosaic Analysis with Double Markers (MADM) is a method for generating genetically
    mosaic mice, in which sibling mutant and wild-type cells are labeled with different
    fluorescent markers. It is a powerful tool that enables analysis of gene function
    at the single cell level in vivo. It requires transgenic cassettes to be located
    between the centromere and the mutation in the gene of interest on the same chromosome.
    Here we compare procedures for introduction of MADM cassettes into new loci in
    the mouse genome, and describe new approaches for expanding the utility of MADM.
    We show that: 1) Targeted homologous recombination outperforms random transgenesis
    in generation of reliably expressed MADM cassettes, 2) MADM cassettes in new genomic
    loci need to be validated for biallelic and ubiquitous expression, 3) Recombination
    between MADM cassettes on different chromosomes can be used to study reciprocal
    chromosomal deletions/duplications, and 4) MADM can be modified to permit transgene
    expression by combining it with a binary expression system. The advances described
    in this study expand current, and enable new and more versatile applications of
    MADM.'
acknowledgement: This work was supported by a National Institutes of Health grant
  to LL (R01-NS050835). BT was a Damon Runyon Fellow and was supported by the Damon
  Runyon Cancer Research Foundation Grant DRG-1819-04. KM was supported by the Japan
  Society for the Promotion of Science program for Research Abroad and Human Frontier
  Science Program Organization (LT00300/2007-L). SH was supported by postdoctoral
  fellowships from the European Molecular Biology Organization (ALTF 851-2005), Human
  Frontier Science Program Organization (LT00805/2006-L), and Swiss National Science
  Foundation (PA00P3_124160 and PA00P3_136482). HZ is a Pew Scholar in Biomedical
  Sciences, supported by The Pew Charitable Trusts. LL is an investigator of the Howard
  Hughes Medical Institute. The funders had no role in study design, data collection
  and analysis, decision to publish, or preparation of the manuscript.
author:
- first_name: Bosiljka
  full_name: Tasic, Bosiljka
  last_name: Tasic
- first_name: Kazunari
  full_name: Miyamichi, Kazunari
  last_name: Miyamichi
- first_name: Simon
  full_name: Simon Hippenmeyer
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Vardhan
  full_name: Dani, Vardhan S.
  last_name: Dani
- first_name: H.
  full_name: Zeng, H.
  last_name: Zeng
- first_name: William
  full_name: Joo, William
  last_name: Joo
- first_name: Hui
  full_name: Zong, Hui
  last_name: Zong
- first_name: Yanru
  full_name: Chen-Tsai, Yanru
  last_name: Chen Tsai
- first_name: Liqun
  full_name: Luo, Liqun
  last_name: Luo
citation:
  ama: Tasic B, Miyamichi K, Hippenmeyer S, et al. Extensions of MADM (Mosaic Analysis
    with Double Markers) in Mice . <i>PLoS One</i>. 2012;7(3). doi:<a href="https://doi.org/10.1371/journal.pone.0033332">10.1371/journal.pone.0033332</a>
  apa: Tasic, B., Miyamichi, K., Hippenmeyer, S., Dani, V., Zeng, H., Joo, W., … Luo,
    L. (2012). Extensions of MADM (Mosaic Analysis with Double Markers) in Mice .
    <i>PLoS One</i>. Public Library of Science. <a href="https://doi.org/10.1371/journal.pone.0033332">https://doi.org/10.1371/journal.pone.0033332</a>
  chicago: Tasic, Bosiljka, Kazunari Miyamichi, Simon Hippenmeyer, Vardhan Dani, H.
    Zeng, William Joo, Hui Zong, Yanru Chen Tsai, and Liqun Luo. “Extensions of MADM
    (Mosaic Analysis with Double Markers) in Mice .” <i>PLoS One</i>. Public Library
    of Science, 2012. <a href="https://doi.org/10.1371/journal.pone.0033332">https://doi.org/10.1371/journal.pone.0033332</a>.
  ieee: B. Tasic <i>et al.</i>, “Extensions of MADM (Mosaic Analysis with Double Markers)
    in Mice ,” <i>PLoS One</i>, vol. 7, no. 3. Public Library of Science, 2012.
  ista: Tasic B, Miyamichi K, Hippenmeyer S, Dani V, Zeng H, Joo W, Zong H, Chen Tsai
    Y, Luo L. 2012. Extensions of MADM (Mosaic Analysis with Double Markers) in Mice
    . PLoS One. 7(3).
  mla: Tasic, Bosiljka, et al. “Extensions of MADM (Mosaic Analysis with Double Markers)
    in Mice .” <i>PLoS One</i>, vol. 7, no. 3, Public Library of Science, 2012, doi:<a
    href="https://doi.org/10.1371/journal.pone.0033332">10.1371/journal.pone.0033332</a>.
  short: B. Tasic, K. Miyamichi, S. Hippenmeyer, V. Dani, H. Zeng, W. Joo, H. Zong,
    Y. Chen Tsai, L. Luo, PLoS One 7 (2012).
date_created: 2018-12-11T11:56:38Z
date_published: 2012-03-27T00:00:00Z
date_updated: 2021-01-12T06:56:22Z
day: '27'
doi: 10.1371/journal.pone.0033332
extern: 1
intvolume: '         7'
issue: '3'
month: '03'
publication: PLoS One
publication_status: published
publisher: Public Library of Science
publist_id: '4683'
quality_controlled: 0
status: public
title: 'Extensions of MADM (Mosaic Analysis with Double Markers) in Mice '
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
volume: 7
year: '2012'
...