---
_id: '2263'
abstract:
- lang: eng
  text: Nestin-cre transgenic mice have been widely used to direct recombination to
    neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs). Here
    we report that a readily utilized, and the only commercially available, Nestin-cre
    line is insufficient for directing recombination in early embryonic NSCs and NPCs.
    Analysis of recombination efficiency in multiple cre-dependent reporters and a
    genetic mosaic line revealed consistent temporal and spatial patterns of recombination
    in NSCs and NPCs. For comparison we utilized a knock-in Emx1cre line and found
    robust recombination in NSCs and NPCs in ventricular and subventricular zones
    of the cerebral cortices as early as embryonic day 12.5. In addition we found
    that the rate of Nestin-cre driven recombination only reaches sufficiently high
    levels in NSCs and NPCs during late embryonic and early postnatal periods. These
    findings are important when commercially available cre lines are considered for
    directing recombination to embryonic NSCs and NPCs.
article_processing_charge: No
author:
- first_name: Huixuan
  full_name: Liang, Huixuan
  last_name: Liang
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: H.
  full_name: Ghashghaei, H.
  last_name: Ghashghaei
citation:
  ama: Liang H, Hippenmeyer S, Ghashghaei H. A Nestin-cre transgenic mouse is insufficient
    for recombination in early embryonic neural progenitors. <i>Biology Open</i>.
    2012;1(12):1200-1203. doi:<a href="https://doi.org/10.1242/bio.20122287">10.1242/bio.20122287</a>
  apa: Liang, H., Hippenmeyer, S., &#38; Ghashghaei, H. (2012). A Nestin-cre transgenic
    mouse is insufficient for recombination in early embryonic neural progenitors.
    <i>Biology Open</i>. Company of Biologists. <a href="https://doi.org/10.1242/bio.20122287">https://doi.org/10.1242/bio.20122287</a>
  chicago: Liang, Huixuan, Simon Hippenmeyer, and H. Ghashghaei. “A Nestin-Cre Transgenic
    Mouse Is Insufficient for Recombination in Early Embryonic Neural Progenitors.”
    <i>Biology Open</i>. Company of Biologists, 2012. <a href="https://doi.org/10.1242/bio.20122287">https://doi.org/10.1242/bio.20122287</a>.
  ieee: H. Liang, S. Hippenmeyer, and H. Ghashghaei, “A Nestin-cre transgenic mouse
    is insufficient for recombination in early embryonic neural progenitors,” <i>Biology
    Open</i>, vol. 1, no. 12. Company of Biologists, pp. 1200–1203, 2012.
  ista: Liang H, Hippenmeyer S, Ghashghaei H. 2012. A Nestin-cre transgenic mouse
    is insufficient for recombination in early embryonic neural progenitors. Biology
    Open. 1(12), 1200–1203.
  mla: Liang, Huixuan, et al. “A Nestin-Cre Transgenic Mouse Is Insufficient for Recombination
    in Early Embryonic Neural Progenitors.” <i>Biology Open</i>, vol. 1, no. 12, Company
    of Biologists, 2012, pp. 1200–03, doi:<a href="https://doi.org/10.1242/bio.20122287">10.1242/bio.20122287</a>.
  short: H. Liang, S. Hippenmeyer, H. Ghashghaei, Biology Open 1 (2012) 1200–1203.
date_created: 2018-12-11T11:56:38Z
date_published: 2012-12-15T00:00:00Z
date_updated: 2026-08-12T09:32:12Z
day: '15'
ddc:
- '576'
department:
- _id: SiHi
doi: 10.1242/bio.20122287
external_id:
  isi:
  - '000209205700005'
file:
- access_level: open_access
  checksum: 605a1800b81227848c361fd6ba7d22ba
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:09Z
  date_updated: 2020-07-14T12:45:35Z
  file_id: '4990'
  file_name: IST-2015-387-v1+1_1200.full.pdf
  file_size: 726695
  relation: main_file
file_date_updated: 2020-07-14T12:45:35Z
fulldoi: https://doi.org/10.1242/bio.20122287
has_accepted_license: '1'
intvolume: '         1'
isi: 1
issue: '12'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 1200 - 1203
publication: Biology Open
publication_status: published
publisher: Company of Biologists
publist_id: '4682'
pubrep_id: '387'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic
  neural progenitors
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
    BY-NC-SA 4.0)
  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2012'
...