---
res:
  bibo_abstract:
  - The one-bond proton-carbon coupling constant (1JCH) is an insightful probe of
    carbohydrate configuration. Equatorial and axial protons at the C1 position typically
    exhibit distinct 1JCH values, enabling NMR measurements to distinguish α- and
    β-configurations in carbohydrates. In principle, such measurements could provide
    insights into carbohydrates in the cell walls of intact microbes. However, traditionally,
    these measurements are performed by solution NMR with carbohydrates that were
    extracted, solubilized and fractionated, leaving the biological relevance of the
    measurements uncertain. Here, we demonstrate that 1H-detected solid-state NMR
    with fast magic-angle spinning allows quantitative measurements of 1JCH couplings
    for mobile capsular polysaccharides, directly on submilligram amounts of pathogenic
    cells. Our approach is demonstrated on intact cells of the pathogenic yeast Cryptococcus
    neoformans. High-resolution proton-detected spectra enabled the determination
    of coupling constants for five mobile polysaccharide units of the cryptococcal
    capsule, revealing their native configurations and confirming previous solution
    NMR-based anomeric configuration assignments.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Alons
      foaf_name: Lends, Alons
      foaf_surname: Lends
  - foaf_Person:
      foaf_givenName: Gaelle
      foaf_name: Lamon, Gaelle
      foaf_surname: Lamon
  - foaf_Person:
      foaf_givenName: Alicia
      foaf_name: Vallet, Alicia
      foaf_surname: Vallet
  - foaf_Person:
      foaf_givenName: Axelle
      foaf_name: Grélard, Axelle
      foaf_surname: Grélard
  - foaf_Person:
      foaf_givenName: Estelle
      foaf_name: Morvan, Estelle
      foaf_surname: Morvan
  - foaf_Person:
      foaf_givenName: Vishukumar
      foaf_name: Aimanianda, Vishukumar
      foaf_surname: Aimanianda
  - foaf_Person:
      foaf_givenName: Paul
      foaf_name: Schanda, Paul
      foaf_surname: Schanda
      foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425
    orcid: 0000-0002-9350-7606
  - foaf_Person:
      foaf_givenName: Antoine
      foaf_name: Loquet, Antoine
      foaf_surname: Loquet
  bibo_doi: 10.1021/jacs.6c06064
  bibo_issue: '27'
  bibo_volume: 148
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0002-7863
  - http://id.crossref.org/issn/1520-5126
  dct_language: eng
  dct_publisher: American Chemical Society@
  dct_title: On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected
    solid-state NMR@
  fabio_hasPubmedId: '42377973'
...