On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR Lends, Alons Lamon, Gaelle Vallet, Alicia Grélard, Axelle Morvan, Estelle Aimanianda, Vishukumar Schanda, Paul ; https://orcid.org/0000-0002-9350-7606 Loquet, Antoine The one-bond proton-carbon coupling constant (1JCH) is an insightful probe of carbohydrate configuration. Equatorial and axial protons at the C1 position typically exhibit distinct 1JCH values, enabling NMR measurements to distinguish α- and β-configurations in carbohydrates. In principle, such measurements could provide insights into carbohydrates in the cell walls of intact microbes. However, traditionally, these measurements are performed by solution NMR with carbohydrates that were extracted, solubilized and fractionated, leaving the biological relevance of the measurements uncertain. Here, we demonstrate that 1H-detected solid-state NMR with fast magic-angle spinning allows quantitative measurements of 1JCH couplings for mobile capsular polysaccharides, directly on submilligram amounts of pathogenic cells. Our approach is demonstrated on intact cells of the pathogenic yeast Cryptococcus neoformans. High-resolution proton-detected spectra enabled the determination of coupling constants for five mobile polysaccharide units of the cryptococcal capsule, revealing their native configurations and confirming previous solution NMR-based anomeric configuration assignments. American Chemical Society 2026 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/22638 Lends A, Lamon G, Vallet A, et al. On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR. <i>Journal of the American Chemical Society</i>. 2026;148(27):28037-28042. doi:<a href="https://doi.org/10.1021/jacs.6c06064">10.1021/jacs.6c06064</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1021/jacs.6c06064 info:eu-repo/semantics/altIdentifier/issn/0002-7863 info:eu-repo/semantics/altIdentifier/e-issn/1520-5126 info:eu-repo/semantics/altIdentifier/pmid/42377973 info:eu-repo/semantics/closedAccess