article published yes Alons Lends author Gaelle Lamon author Alicia Vallet author Axelle Grélard author Estelle Morvan author Vishukumar Aimanianda author Paul Schanda author 7B541462-FAF6-11E9-A490-E8DFE56974250000-0002-9350-7606 Antoine Loquet author PaSc department The one-bond proton-carbon coupling constant (1JCH) is an insightful probe of carbohydrate configuration. Equatorial and axial protons at the C1 position typically exhibit distinct 1JCH values, enabling NMR measurements to distinguish α- and β-configurations in carbohydrates. In principle, such measurements could provide insights into carbohydrates in the cell walls of intact microbes. However, traditionally, these measurements are performed by solution NMR with carbohydrates that were extracted, solubilized and fractionated, leaving the biological relevance of the measurements uncertain. Here, we demonstrate that 1H-detected solid-state NMR with fast magic-angle spinning allows quantitative measurements of 1JCH couplings for mobile capsular polysaccharides, directly on submilligram amounts of pathogenic cells. Our approach is demonstrated on intact cells of the pathogenic yeast Cryptococcus neoformans. High-resolution proton-detected spectra enabled the determination of coupling constants for five mobile polysaccharide units of the cryptococcal capsule, revealing their native configurations and confirming previous solution NMR-based anomeric configuration assignments. American Chemical Society2026 eng 0002-7863 1520-5126 4237797310.1021/jacs.6c06064 1482728037-28042 Lends, A., Lamon, G., Vallet, A., Grélard, A., Morvan, E., Aimanianda, V., … Loquet, A. (2026). On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href="https://doi.org/10.1021/jacs.6c06064">https://doi.org/10.1021/jacs.6c06064</a> Lends, Alons, Gaelle Lamon, Alicia Vallet, Axelle Grélard, Estelle Morvan, Vishukumar Aimanianda, Paul Schanda, and Antoine Loquet. “On-Cell Detection of Polysaccharide One-Bond1Jch Couplings by Proton-Detected Solid-State NMR.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2026. <a href="https://doi.org/10.1021/jacs.6c06064">https://doi.org/10.1021/jacs.6c06064</a>. Lends A, Lamon G, Vallet A, et al. On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR. <i>Journal of the American Chemical Society</i>. 2026;148(27):28037-28042. doi:<a href="https://doi.org/10.1021/jacs.6c06064">10.1021/jacs.6c06064</a> A. Lends, G. Lamon, A. Vallet, A. Grélard, E. Morvan, V. Aimanianda, P. Schanda, A. Loquet, Journal of the American Chemical Society 148 (2026) 28037–28042. Lends A, Lamon G, Vallet A, Grélard A, Morvan E, Aimanianda V, Schanda P, Loquet A. 2026. On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR. Journal of the American Chemical Society. 148(27), 28037–28042. Lends, Alons, et al. “On-Cell Detection of Polysaccharide One-Bond1Jch Couplings by Proton-Detected Solid-State NMR.” <i>Journal of the American Chemical Society</i>, vol. 148, no. 27, American Chemical Society, 2026, pp. 28037–42, doi:<a href="https://doi.org/10.1021/jacs.6c06064">10.1021/jacs.6c06064</a>. A. Lends <i>et al.</i>, “On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected solid-state NMR,” <i>Journal of the American Chemical Society</i>, vol. 148, no. 27. American Chemical Society, pp. 28037–28042, 2026. 226382026-08-03T13:20:41Z2026-08-04T05:52:13Z