---
OA_type: closed access
_id: '22638'
abstract:
- lang: eng
  text: The one-bond proton-carbon coupling constant (1JCH) is an insightful probe
    of carbohydrate configuration. Equatorial and axial protons at the C1 position
    typically exhibit distinct 1JCH values, enabling NMR measurements to distinguish
    α- and β-configurations in carbohydrates. In principle, such measurements could
    provide insights into carbohydrates in the cell walls of intact microbes. However,
    traditionally, these measurements are performed by solution NMR with carbohydrates
    that were extracted, solubilized and fractionated, leaving the biological relevance
    of the measurements uncertain. Here, we demonstrate that 1H-detected solid-state
    NMR with fast magic-angle spinning allows quantitative measurements of 1JCH couplings
    for mobile capsular polysaccharides, directly on submilligram amounts of pathogenic
    cells. Our approach is demonstrated on intact cells of the pathogenic yeast Cryptococcus
    neoformans. High-resolution proton-detected spectra enabled the determination
    of coupling constants for five mobile polysaccharide units of the cryptococcal
    capsule, revealing their native configurations and confirming previous solution
    NMR-based anomeric configuration assignments.
acknowledgement: We thank the ANR (ANR-16-CE11-0020-02 to A. Loquet and V.A. and ANR-21-CE17-0032
  to V.A.) as well as the Swiss National Science Foundation for early postdoc mobility
  project P2EZP2_184258 to A. Lends. This work has benefited from the Biophysical
  and Structural Chemistry Platform at Institut Européen de Chimie et Biologie IECB,
  Centre National de la Recherche Scientifique CNRS Unité d’Appui et de Recherche
  UAR 3033, INSERM US001, and the CNRS (IR-RMN FR3050 and Infranalytics FR2054).
article_processing_charge: No
article_type: original
author:
- first_name: Alons
  full_name: Lends, Alons
  last_name: Lends
- first_name: Gaelle
  full_name: Lamon, Gaelle
  last_name: Lamon
- first_name: Alicia
  full_name: Vallet, Alicia
  last_name: Vallet
- first_name: Axelle
  full_name: Grélard, Axelle
  last_name: Grélard
- first_name: Estelle
  full_name: Morvan, Estelle
  last_name: Morvan
- first_name: Vishukumar
  full_name: Aimanianda, Vishukumar
  last_name: Aimanianda
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Antoine
  full_name: Loquet, Antoine
  last_name: Loquet
citation:
  ama: Lends A, Lamon G, Vallet A, et al. On-cell detection of polysaccharide one-bond1Jch
    couplings by proton-detected solid-state NMR. <i>Journal of the American Chemical
    Society</i>. 2026;148(27):28037-28042. doi:<a href="https://doi.org/10.1021/jacs.6c06064">10.1021/jacs.6c06064</a>
  apa: Lends, A., Lamon, G., Vallet, A., Grélard, A., Morvan, E., Aimanianda, V.,
    … Loquet, A. (2026). On-cell detection of polysaccharide one-bond1Jch couplings
    by proton-detected solid-state NMR. <i>Journal of the American Chemical Society</i>.
    American Chemical Society. <a href="https://doi.org/10.1021/jacs.6c06064">https://doi.org/10.1021/jacs.6c06064</a>
  chicago: Lends, Alons, Gaelle Lamon, Alicia Vallet, Axelle Grélard, Estelle Morvan,
    Vishukumar Aimanianda, Paul Schanda, and Antoine Loquet. “On-Cell Detection of
    Polysaccharide One-Bond1Jch Couplings by Proton-Detected Solid-State NMR.” <i>Journal
    of the American Chemical Society</i>. American Chemical Society, 2026. <a href="https://doi.org/10.1021/jacs.6c06064">https://doi.org/10.1021/jacs.6c06064</a>.
  ieee: A. Lends <i>et al.</i>, “On-cell detection of polysaccharide one-bond1Jch
    couplings by proton-detected solid-state NMR,” <i>Journal of the American Chemical
    Society</i>, vol. 148, no. 27. American Chemical Society, pp. 28037–28042, 2026.
  ista: Lends A, Lamon G, Vallet A, Grélard A, Morvan E, Aimanianda V, Schanda P,
    Loquet A. 2026. On-cell detection of polysaccharide one-bond1Jch couplings by
    proton-detected solid-state NMR. Journal of the American Chemical Society. 148(27),
    28037–28042.
  mla: Lends, Alons, et al. “On-Cell Detection of Polysaccharide One-Bond1Jch Couplings
    by Proton-Detected Solid-State NMR.” <i>Journal of the American Chemical Society</i>,
    vol. 148, no. 27, American Chemical Society, 2026, pp. 28037–42, doi:<a href="https://doi.org/10.1021/jacs.6c06064">10.1021/jacs.6c06064</a>.
  short: A. Lends, G. Lamon, A. Vallet, A. Grélard, E. Morvan, V. Aimanianda, P. Schanda,
    A. Loquet, Journal of the American Chemical Society 148 (2026) 28037–28042.
das_tickbox: '0'
date_created: 2026-08-03T13:20:41Z
date_published: 2026-06-15T00:00:00Z
date_updated: 2026-08-04T05:52:13Z
day: '15'
department:
- _id: PaSc
doi: 10.1021/jacs.6c06064
external_id:
  pmid:
  - '42377973'
intvolume: '       148'
issue: '27'
language:
- iso: eng
month: '06'
oa_version: None
page: 28037-28042
pmid: 1
publication: Journal of the American Chemical Society
publication_identifier:
  eissn:
  - 1520-5126
  issn:
  - 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: On-cell detection of polysaccharide one-bond1Jch couplings by proton-detected
  solid-state NMR
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 148
year: '2026'
...