---
OA_place: publisher
OA_type: hybrid
_id: '22647'
abstract:
- lang: eng
  text: "Within the plant endomembrane system, the vesicle coat protein clathrin localizes
    to the plasma membrane (PM) and the trans-Golgi Network/early endosome (TGN/EE).
    While the role of clathrin in endocytosis at the PM is well established, its function
    at TGN/EE, presumably in late secretion (trafficking from the TGN/EE to the cell
    surface) or en route to the vacuole, is debated. Similarly debated are potential
    homeostatic mechanisms balancing the trafficking routes, especially endocytosis
    and late secretion.\r\nWe address these questions in Arabidopsis thaliana using
    conditional silencing of CLATHRIN HEAVY CHAIN (CHC), conditional overexpression
    of the clathrin uncoating factor AUXILIN-LIKE1, and secretory mutants.\r\nCHC
    silencing interferes with trafficking of cargoes destined for the apoplast and
    the PM, supporting a function of clathrin in late secretion. The secretory cargoes
    become abnormally rerouted from the TGN/EE to the vacuole. Unlike CHC silencing,
    overexpression of AUXILIN-LIKE1 selectively inhibits clathrin-mediated endocytosis
    while secretion continues normally at early points of induction. Conversely, secretory
    mutants exhibit a reduced PM recruitment of clathrin, and variably, of the TPLATE
    endocytic component.\r\nTogether, our data show a role of clathrin in secretion
    and suggest secretion as a fundamental trafficking process to which endocytosis
    is adjusted by a weak homeostatic mechanism."
acknowledgement: 'The authors wish to acknowledge Dr. Paweł Baster for cloning PIN1-GFP-2/pDONR221,
  Ms. Aline Monzer and Dr. Mingyue Li for help with CHC protein level evaluation,
  Dr. Michał Rychłowski for help with confocal microscopy, Prof. Ari Pekka Mähönen
  for sharing the p1R4-pUBQ10:XVE plasmid, and Prof. Ying Gu for sharing seeds of
  the sec5 mutant. M.A. would like to thank Dr. Xixi Zhang and Prof. Sebastian Bednarek
  for inspiring discussions. This work was supported by the Taif University Researchers
  Supporting Project, TURSP-HC2022/02 to JF and SA and Austrian Science Fund (FWF):
  I 3630-B25 to JF. Open Access funding provided by Institute of Science and Technology
  Austria.'
article_number: nph.71454
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Adam
  full_name: Gackowski, Adam
  last_name: Gackowski
- first_name: Ivana
  full_name: Matijevic, Ivana
  id: 83c17ce3-15b2-11ec-abd3-f486545870bd
  last_name: Matijevic
- first_name: Saqer S.
  full_name: Alotaibi, Saqer S.
  last_name: Alotaibi
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. The role of clathrin
    in post‐Golgi secretion in plant cells. <i>New Phytologist</i>. 2026. doi:<a href="https://doi.org/10.1111/nph.71454">10.1111/nph.71454</a>
  apa: Adamowski, M., Gackowski, A., Matijevic, I., Alotaibi, S. S., &#38; Friml,
    J. (2026). The role of clathrin in post‐Golgi secretion in plant cells. <i>New
    Phytologist</i>. Wiley. <a href="https://doi.org/10.1111/nph.71454">https://doi.org/10.1111/nph.71454</a>
  chicago: Adamowski, Maciek, Adam Gackowski, Ivana Matijevic, Saqer S. Alotaibi,
    and Jiří Friml. “The Role of Clathrin in Post‐Golgi Secretion in Plant Cells.”
    <i>New Phytologist</i>. Wiley, 2026. <a href="https://doi.org/10.1111/nph.71454">https://doi.org/10.1111/nph.71454</a>.
  ieee: M. Adamowski, A. Gackowski, I. Matijevic, S. S. Alotaibi, and J. Friml, “The
    role of clathrin in post‐Golgi secretion in plant cells,” <i>New Phytologist</i>.
    Wiley, 2026.
  ista: Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. 2026. The role
    of clathrin in post‐Golgi secretion in plant cells. New Phytologist., nph. 71454.
  mla: Adamowski, Maciek, et al. “The Role of Clathrin in Post‐Golgi Secretion in
    Plant Cells.” <i>New Phytologist</i>, nph. 71454, Wiley, 2026, doi:<a href="https://doi.org/10.1111/nph.71454">10.1111/nph.71454</a>.
  short: M. Adamowski, A. Gackowski, I. Matijevic, S.S. Alotaibi, J. Friml, New Phytologist
    (2026).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: 'Original data associated with this study have been deposited
  in Dataset S1. The accession nos. of A. thaliana genes used in this study are as
  follows: CHC1 (AT3G11130), CHC2 (AT3G08530), CLC2 (AT2G40060), TPLATE (AT3G01780),
  AP2A1 (AT5G22770), DRP1C (AT1G14830), GNOM-LIKE1 (AT5G39500), BEN3/BIG2 (AT3G60860),
  TMK4 (AT3G23750), PIN1 (AT1G73590), AUXILIN-LIKE1 (AT4G12780), AP1M2 (AT1G60780),
  ECHIDNA (AT1G09330), SEC5A (AT1G76850), SEC5B (AT1G21170), TUB2 (AT5G62690), and
  PP2AA3 (AT1G13320).'
date_created: 2026-08-04T06:48:41Z
date_published: 2026-07-20T00:00:00Z
date_updated: 2026-08-04T07:58:27Z
day: '20'
department:
- _id: JiFr
- _id: MaLo
- _id: GradSch
doi: 10.1111/nph.71454
external_id:
  pmid:
  - '42477503'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1111/nph.71454
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: New Phytologist
publication_identifier:
  eissn:
  - 1469-8137
  issn:
  - 0028-646X
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: The role of clathrin in post‐Golgi secretion in plant cells
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...