---
res:
  bibo_abstract:
  - Sleep need is associated with both circuit dynamics and widespread synaptic plasticity,
    yet the specific synaptic changes underlying sleep homeostasis remain incompletely
    understood. In Drosophila, sleep loss has been shown to trigger plasticity of
    the presynaptic active zone, marked by increasing levels of the ELKS-family scaffold
    protein Bruchpilot (BRP). By titrating brp gene copy number, we previously established
    a presynapse-specific, dosage-dependent paradigm that modulates sleep pressure.
    Here, to elucidate the molecular landscape of this plasticity, we performed synapse-enriched
    integrated-omics. Proteomic and bioinformatic analyses revealed changes in immune
    and stress response pathways and local translation control. Strikingly, phospho-proteomic
    analysis uncovered a global shift toward hypophosphorylation, particularly in
    presynaptic proteins, indicating a reprogramming of the phosphorylation–dephosphorylation
    balance. This presynaptic hypophosphorylation is likely contributed by reduced
    activity of Protein Kinase A (PKA) and enhanced substrate affinity of Protein
    Phosphatase 1 (PP1) mediated by its regulatory subunit Spinophilin (Spn). Manipulating
    either PKA or PP1 activity was sufficient to suppress BRP-modulated sleep phenotypes.
    We propose that presynaptic hypophosphorylation constitutes a molecular signature
    of local synaptic remodeling that adaptively tunes sleep need via reversible posttranslational
    modification, a mechanism likely conserved across species.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Chengji
      foaf_name: Piao, Chengji
      foaf_surname: Piao
  - foaf_Person:
      foaf_givenName: Ewelina
      foaf_name: Dutkiewicz, Ewelina
      foaf_surname: Dutkiewicz
      foaf_workInfoHomepage: http://www.librecat.org/personId=0601cc46-c082-11ec-9b07-bb29641d1de9
  - foaf_Person:
      foaf_givenName: Laxmikanth
      foaf_name: Kollipara, Laxmikanth
      foaf_surname: Kollipara
  - foaf_Person:
      foaf_givenName: Albert
      foaf_name: Sickmann, Albert
      foaf_surname: Sickmann
  - foaf_Person:
      foaf_givenName: Sheng
      foaf_name: Huang, Sheng
      foaf_surname: Huang
  - foaf_Person:
      foaf_givenName: Stephan J.
      foaf_name: Sigrist, Stephan J.
      foaf_surname: Sigrist
  bibo_doi: 10.1073/pnas.2524065123
  bibo_issue: '24'
  bibo_volume: 123
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0027-8424
  - http://id.crossref.org/issn/1091-6490
  dct_language: eng
  dct_publisher: National Academy of Sciences@
  dct_title: Active zone plasticity couples sleep need to presynaptic hypophosphorylation@
  fabio_hasPubmedId: '42258713'
...