---
OA_place: repository
OA_type: green
_id: '22803'
abstract:
- lang: eng
  text: 'In this study, we analyzed the efficiency of different adeno-associated viral
    (AAV) injections in transfecting neurons in the salamander Pleurodeles waltl.
    To query sources of variation in AAV injection outcomes, we analyzed metadata
    and outcomes for an additional 68 intraparenchymal injections performed 39 in
    post-metamorphic Pleurodeles salamanders. This dataset included both quantitative
    variables (age, weight, viral genomes (v.g.) injected, and a cell transduction
    score ranging from 0 to 4, see STAR Methods) and categorical variables (here referred
    to as qualitative variables: serotype, promoter, reporter, single vs. dual injection,
    manufacturer, and injection site). To assess the associations between these variables,
    we performed a Factor Analysis for Mixed Data (FAMD), a principal component method
    that is designed to determine significant sources of variability within datasets
    that contain both quantitative and qualitative data types. This dataset contains
    the injection outcomes and metadata for AAV injections administered in the salamander
    Pleurodeles waltl. The RactoMineR package (https://CRAN.R-project.org/package=FactoMineR)
    was used to determine significant contributions of a number of variables contributing
    to injection outcomes. Analysis of these data revealed that, among other factors,
    age co-varies with injection score. Therefore, we conclude that increased animal
    age decreases the efficacy of this tool.'
article_processing_charge: No
author:
- first_name: Eliza
  full_name: Jaeger, Eliza
  last_name: Jaeger
citation:
  ama: Jaeger E. Multivariate analysis of Pleurodeles waltl injection outcomes using
    FAMD FactoMineR (RStudio 4.3.2) used to probe sources of variability during an
    Adeno-associated viral (AAV) screen. 2024. doi:<a href="https://doi.org/10.5061/dryad.mpg4f4r89">10.5061/dryad.mpg4f4r89</a>
  apa: Jaeger, E. (2024). Multivariate analysis of Pleurodeles waltl injection outcomes
    using FAMD FactoMineR (RStudio 4.3.2) used to probe sources of variability during
    an Adeno-associated viral (AAV) screen. Repository. <a href="https://doi.org/10.5061/dryad.mpg4f4r89">https://doi.org/10.5061/dryad.mpg4f4r89</a>
  chicago: Jaeger, Eliza. “Multivariate Analysis of Pleurodeles Waltl Injection Outcomes
    Using FAMD FactoMineR (RStudio 4.3.2) Used to Probe Sources of Variability during
    an Adeno-Associated Viral (AAV) Screen.” Repository, 2024. <a href="https://doi.org/10.5061/dryad.mpg4f4r89">https://doi.org/10.5061/dryad.mpg4f4r89</a>.
  ieee: E. Jaeger, “Multivariate analysis of Pleurodeles waltl injection outcomes
    using FAMD FactoMineR (RStudio 4.3.2) used to probe sources of variability during
    an Adeno-associated viral (AAV) screen.” Repository, 2024.
  ista: Jaeger E. 2024. Multivariate analysis of Pleurodeles waltl injection outcomes
    using FAMD FactoMineR (RStudio 4.3.2) used to probe sources of variability during
    an Adeno-associated viral (AAV) screen, Repository, <a href="https://doi.org/10.5061/dryad.mpg4f4r89">10.5061/dryad.mpg4f4r89</a>.
  mla: Jaeger, Eliza. <i>Multivariate Analysis of Pleurodeles Waltl Injection Outcomes
    Using FAMD FactoMineR (RStudio 4.3.2) Used to Probe Sources of Variability during
    an Adeno-Associated Viral (AAV) Screen</i>. Repository, 2024, doi:<a href="https://doi.org/10.5061/dryad.mpg4f4r89">10.5061/dryad.mpg4f4r89</a>.
  short: E. Jaeger, (2024).
date_created: 2026-09-04T10:10:52Z
date_published: 2024-10-10T00:00:00Z
date_updated: 2026-09-04T10:11:04Z
day: '10'
ddc:
- '570'
department:
- _id: E-Lib
doi: 10.5061/dryad.mpg4f4r89
main_file_link:
- open_access: '1'
  url: https://doi.org/10.5061/dryad.mpg4f4r89
month: '10'
oa: 1
oa_version: None
publisher: Repository
related_material:
  record:
  - id: '15016'
    relation: used_in_publication
    status: public
status: public
title: Multivariate analysis of Pleurodeles waltl injection outcomes using FAMD FactoMineR
  (RStudio 4.3.2) used to probe sources of variability during an Adeno-associated
  viral (AAV) screen
type: research_data_reference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...