---
res:
  bibo_abstract:
  - Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed detection
    of mRNA with subcellular spatial resolution. It employs fluorophore-tagged DNA
    hairpins that self-assemble on target-bound probe pairs, amplifying the signal
    without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence
    in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated
    image analysis pipeline that enables per-cell quantification of probe expression
    and co-localization. This method provides a robust and quantitative approach for
    visualizing gene expression patterns in situ.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: David
      foaf_name: Vijatovic, David
      foaf_surname: Vijatovic
      foaf_workInfoHomepage: http://www.librecat.org/personId=cf391e77-ec3c-11ea-a124-d69323410b58
    orcid: 0000-0002-5494-0941
  - foaf_Person:
      foaf_givenName: Stavros
      foaf_name: Papadopoulos, Stavros
      foaf_surname: Papadopoulos
      foaf_workInfoHomepage: http://www.librecat.org/personId=40606b92-f128-11eb-9611-bf66a98cfa5c
  - foaf_Person:
      foaf_givenName: Marco
      foaf_name: Dalla Vecchia, Marco
      foaf_surname: Dalla Vecchia
      foaf_workInfoHomepage: http://www.librecat.org/personId=02a7a869-ff06-11ed-a87f-86649d6077e5
  - foaf_Person:
      foaf_givenName: Lora Beatrice Jaeger
      foaf_name: Sweeney, Lora Beatrice Jaeger
      foaf_surname: Sweeney
      foaf_workInfoHomepage: http://www.librecat.org/personId=56BE8254-C4F0-11E9-8E45-0B23E6697425
    orcid: 0000-0001-9242-5601
  bibo_doi: 10.1007/978-1-0716-5360-9_11
  bibo_volume: 3049
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1940-6029
  dct_language: eng
  dct_publisher: Springer@
  dct_subject:
  - mRNA FISH
  - Hybridization chain reaction
  - In situ hybridization
  - Fluorescence microscopy
  dct_title: Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain
    Reaction (HCR) in Xenopus cryosections@
  fabio_hasPubmedId: '42681228'
...