[{"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"intvolume":"      3049","has_accepted_license":"1","external_id":{"pmid":["42681226"]},"month":"09","quality_controlled":"1","das_tickbox":"0","file_date_updated":"2026-09-17T08:00:45Z","type":"book_chapter","title":"Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus","status":"public","publisher":"Springer","publication_status":"published","OA_type":"hybrid","date_published":"2026-09-02T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2026-09-13T22:01:56Z","OA_place":"publisher","_id":"22926","publication":"Xenopus","day":"02","page":"219-229","pmid":1,"oa":1,"language":[{"iso":"eng"}],"supplementarymaterial":"no","author":[{"orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","first_name":"David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"date_updated":"2026-09-17T08:03:32Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.1007/978-1-0716-5360-9_9","corr_author":"1","researchdata_availability":"no","series_title":"MIMB","file":[{"date_created":"2026-09-17T08:00:45Z","checksum":"ea5af1374dd6820830773e0e96535ac0","access_level":"open_access","file_size":852042,"date_updated":"2026-09-17T08:00:45Z","creator":"dernst","file_id":"22939","file_name":"2026_MIMB_Vijatovic2.pdf","content_type":"application/pdf","success":1,"relation":"main_file"}],"editor":[{"last_name":"Beck","first_name":"Caroline W.","full_name":"Beck, Caroline W."}],"publication_identifier":{"eissn":["1940-6029"]},"project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"grant_number":"F7814","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity"},{"name":"Neuronal circuits in health and disease (Sweeney)","grant_number":"COE16","_id":"cf428362-b037-11f1-b015-8277a8a2f63d"},{"_id":"34a02c70-11ca-11ed-8bc3-fbfd2c86c88f","grant_number":"3(GG016346-01)","name":"Development of Viral Vectors for Amphibian Gene Delivery and Manipulation"}],"volume":3049,"department":[{"_id":"LoSw"}],"main_file_link":[{"url":"https://doi.org/10.1007/978-1-0716-5360-9_9","open_access":"1"}],"article_processing_charge":"Yes (in subscription journal)","alternative_title":["Methods in Molecular Biology"],"citation":{"ama":"Vijatovic D, Sweeney LB. Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In: Beck CW, ed. <i>Xenopus</i>. Vol 3049. MIMB. Springer; 2026:219-229. doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">10.1007/978-1-0716-5360-9_9</a>","ista":"Vijatovic D, Sweeney LB. 2026.Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In: Xenopus. Methods in Molecular Biology, vol. 3049, 219–229.","apa":"Vijatovic, D., &#38; Sweeney, L. B. (2026). Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In C. W. Beck (Ed.), <i>Xenopus</i> (Vol. 3049, pp. 219–229). Springer. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">https://doi.org/10.1007/978-1-0716-5360-9_9</a>","ieee":"D. Vijatovic and L. B. Sweeney, “Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus,” in <i>Xenopus</i>, vol. 3049, C. W. Beck, Ed. Springer, 2026, pp. 219–229.","mla":"Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus.” <i>Xenopus</i>, edited by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 219–29, doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">10.1007/978-1-0716-5360-9_9</a>.","short":"D. Vijatovic, L.B. Sweeney, in:, C.W. Beck (Ed.), Xenopus, Springer, 2026, pp. 219–229.","chicago":"Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus.” In <i>Xenopus</i>, edited by Caroline W. Beck, 3049:219–29. MIMB. Springer, 2026. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">https://doi.org/10.1007/978-1-0716-5360-9_9</a>."},"abstract":[{"lang":"eng","text":"Adeno-associated viruses (AAVs) provide a versatile tool for labeling neurons in the Xenopus central nervous system across developmental stages. AAV-mediated transgene expression is long-lasting and stable, enabling robust labeling of neuronal populations without genomic integration and without the need to generate transgenic lines. The choice of AAV capsid and promoter can restrict expression to a population of interest, and depending on the route and timing of delivery, AAVs can selectively target either progenitor-derived neuronal cohorts or mature neuronal populations. Here, we have outlined two labeling strategies. First, intraventricular injections in tadpoles transduce neural progenitor cells lining the ventricular system, resulting in cohort-based labeling of neurons as they differentiate during development. Second, direct intraparenchymal injections in post-metamorphic frogs enable spatially restricted labeling and anterograde or retrograde tracing of connectivity within defined brain regions. Together, these approaches provide selective access to developing and mature neural circuits in X. laevis, supporting applications ranging from anatomical tracing to functional imaging and manipulation of neural circuits."}],"year":"2026","acknowledgement":"This work was supported by a Horizon Europe ERC Starting Grant Number 101041551 (L.B.S. and D.V.), FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.), NSF IOS Grant Number 2110086 (L.B.S. and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B (L.B.S.), and Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S. and D.V.).","scopus_import":"1","ddc":["570"],"doi":"10.1007/978-1-0716-5360-9_9"}]