{"title":"Brainstem sensing of multiple body signals during food consumption","day":"17","publication":"Neuron","researchdata_availability":"yes","publication_identifier":{"issn":["0896-6273"],"eissn":["1097-4199"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","fulldoi":"https://doi.org/10.1016/j.neuron.2026.08.015","date_published":"2026-09-17T00:00:00Z","oa":1,"oa_version":"Preprint","_id":"23003","year":"2026","article_type":"original","type":"journal_article","language":[{"iso":"eng"}],"dataavailabilitystatement":"This study did not generate any new, unique reagents.Single-neuron calcium imaging traces from all experiments have been deposited at Mendeley Data at 10.17632/wh6f8xt64y.1 and are publicly available as of the date of publication. Raw two-photon calcium imaging and fiber photometry datasets are available from the lead contact upon request.\r\nAll original code is deposited on GitHub (https://github.com/kmruda/PBN_sensing_body_signals) as well as Zenodo at 10.5281/zenodo.21926666 and is publicly available as of the date of publication.\r\nAny additional information required to reanalyze the data is available upon request from the lead contact, Mark L. Andermann (manderma@bidmc.harvard.edu).","pmid":1,"acknowledgement":"We thank S. Zhang, K. Evans, C. Massengill, J.S. Alvarado, and members of the Andermann lab for helpful discussion and feedback. We thank J. Mathai for the use of the LabScope for fluoroscopy experiments. We thank J. Fernando, P. Prasad, D. Guarino, J. DeBolt, Z. Stolberg, and A. Pinilla for animal husbandry and other technical assistance. Authors were supported by NSF GRFP DGE1745303, NIH F31DC020631, and NIH T32DK007516 (R.A.E.); NIH F32DK135247 and NIH T32DK007516 (K.R.); the BBRF Young Investigator Grant (H.K.); the Research Council of Norway Grant 250259 (S.G. and K.K.L.); and the Pew Innovation Fund, the McKnight Foundation, the Klarman Family Foundation, the Harvard Brain Science Initiative Bipolar Disorder Seed Grant supported by Kent and Liz Dauten, the NIH (DP1AT010971, DP1DK139958, and P01HL149630), a gift from Paul Weisman, and the K. Lisa Yang Brain-Body Center (M.L.A.).","OA_place":"repository","status":"public","das_tickbox":"1","date_created":"2026-09-27T22:01:53Z","OA_type":"green","supplementarymaterial":"yes","external_id":{"pmid":["42753729"],"biorxivid":["10.1101/2025.04.28.651046"]},"quality_controlled":"1","month":"09","date_updated":"2026-10-07T08:40:54Z","publication_status":"epub_ahead","biorxivid":1,"department":[{"_id":"MaJö"}],"article_processing_charge":"No","abstract":[{"text":"The peripheral neural pathways that relay oral and post-ingestive chemo- and mechanosensory signals to the brain during food consumption are increasingly well understood. Far less is known about how these and other body signals are integrated and organized in the brainstem lateral parabrachial nucleus (LPBN), a key interoceptive sensory hub. We established methods for dense calcium imaging of 100s of neurons from mouse LPBN. Food consumption drove a seconds-long wave of activity across LPBN, with dynamics mirroring the movement of food through the upper gastrointestinal tract as observed using X-ray videofluoroscopy. By imaging the same neurons across days, we found that spatially clustered subsets of neurons encoded oral signals, stomach filling, visceral malaise, arousal, and/or body movement. Moreover, only certain subsets were modulated by cortical input. Together, these experiments establish a platform for understanding how peripheral and central interoceptive signals are integrated in the brainstem to guide behavior.","lang":"eng"}],"main_file_link":[{"url":"https://doi.org/10.1101/2025.04.28.651046","open_access":"1"}],"publisher":"Cell Press","doi":"10.1016/j.neuron.2026.08.015","author":[{"full_name":"Essner, Rachel A.","first_name":"Rachel A.","last_name":"Essner"},{"full_name":"Ruda, Kiersten","first_name":"Kiersten","last_name":"Ruda"},{"full_name":"Choh, Hannah J.","first_name":"Hannah J.","last_name":"Choh"},{"last_name":"Kücükdereli","id":"5d5f6ea4-ef9e-11f0-a10a-85e12a3552af","first_name":"Hakan","full_name":"Kücükdereli, Hakan"},{"last_name":"Amsalem","first_name":"Oren","full_name":"Amsalem, Oren"},{"first_name":"Julia","full_name":"Edelhaus, Julia","last_name":"Edelhaus"},{"last_name":"Chahyadinata","first_name":"Gracesenia","full_name":"Chahyadinata, Gracesenia"},{"last_name":"Grødem","first_name":"Sverre","full_name":"Grødem, Sverre"},{"first_name":"Kristian K.","full_name":"Lensjø, Kristian K.","last_name":"Lensjø"},{"first_name":"Teresa E.","full_name":"Lever, Teresa E.","last_name":"Lever"},{"full_name":"Andermann, Mark L.","first_name":"Mark L.","last_name":"Andermann"}],"citation":{"mla":"Essner, Rachel A., et al. “Brainstem Sensing of Multiple Body Signals during Food Consumption.” <i>Neuron</i>, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.08.015\">10.1016/j.neuron.2026.08.015</a>.","chicago":"Essner, Rachel A., Kiersten Ruda, Hannah J. Choh, Hakan Kücükdereli, Oren Amsalem, Julia Edelhaus, Gracesenia Chahyadinata, et al. “Brainstem Sensing of Multiple Body Signals during Food Consumption.” <i>Neuron</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.neuron.2026.08.015\">https://doi.org/10.1016/j.neuron.2026.08.015</a>.","ieee":"R. A. Essner <i>et al.</i>, “Brainstem sensing of multiple body signals during food consumption,” <i>Neuron</i>. Cell Press, 2026.","apa":"Essner, R. A., Ruda, K., Choh, H. J., Kücükdereli, H., Amsalem, O., Edelhaus, J., … Andermann, M. L. (2026). Brainstem sensing of multiple body signals during food consumption. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2026.08.015\">https://doi.org/10.1016/j.neuron.2026.08.015</a>","ama":"Essner RA, Ruda K, Choh HJ, et al. Brainstem sensing of multiple body signals during food consumption. <i>Neuron</i>. 2026. doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.08.015\">10.1016/j.neuron.2026.08.015</a>","short":"R.A. Essner, K. Ruda, H.J. Choh, H. Kücükdereli, O. Amsalem, J. Edelhaus, G. Chahyadinata, S. Grødem, K.K. Lensjø, T.E. Lever, M.L. Andermann, Neuron (2026).","ista":"Essner RA, Ruda K, Choh HJ, Kücükdereli H, Amsalem O, Edelhaus J, Chahyadinata G, Grødem S, Lensjø KK, Lever TE, Andermann ML. 2026. Brainstem sensing of multiple body signals during food consumption. Neuron."}}