---
OA_place: repository
OA_type: green
_id: '23025'
abstract:
- lang: eng
  text: The globally distributed marine alga Emiliania huxleyi produces reflective
    calcite disks (coccoliths) that increase the albedo of ocean water and thus reduce
    the heat absorption in the ocean, which cools the Earth’s climate. The population
    density of E. huxleyi is restricted by Nucleocytoviricota viruses, including E.
    huxleyi virus 201 (EhV-201). Despite the impact of E. huxleyi viruses on the climate,
    there is limited information about their structure and replication. Here we show
    that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane,
    capsid, and outer membrane decorated with numerous transmembrane proteins. The
    virions are prone to deformation, and parts of their capsids deviate from the
    icosahedral arrangement. EhV-201 virions infect E. huxleyi by using their fivefold
    vertex to bind to a host cell and fuse the virus’s inner membrane with the plasma
    membrane. Whereas the replication of EhV-201 probably occurs in the nucleus, virions
    assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane
    segments. Genome packaging initiates synchronously with the capsid assembly and
    completes through an aperture in the forming capsid. Upon the completion of genome
    packaging, the capsids change conformation, which enables them to acquire an outer
    membrane by budding into intracellular vesicles. EhV-201 infection induces a loss
    of surface protective layers from E. huxleyi cells, which allows the continuous
    release of virions by exocytosis. Our results provide insight into how EhVs bypass
    the surface protective layers of E. huxleyi and exploit the organelles of an infected
    cell for progeny assembly.
article_processing_charge: No
author:
- first_name: Miroslav
  full_name: Homola, Miroslav
  last_name: Homola
- first_name: Carina
  full_name: Büttner, Carina
  last_name: Büttner
- first_name: Tibor
  full_name: Füzik, Tibor
  last_name: Füzik
- first_name: Pavel
  full_name: Krepelka, Pavel
  last_name: Krepelka
- first_name: Radka
  full_name: Holbová, Radka
  last_name: Holbová
- first_name: Marten
  full_name: Chaillet, Marten
  last_name: Chaillet
- first_name: Jiří
  full_name: Nováček, Jiří
  last_name: Nováček
- first_name: Friedrich
  full_name: Förster, Friedrich
  last_name: Förster
- first_name: William
  full_name: Wilson, William
  last_name: Wilson
- first_name: Declan
  full_name: Schroeder, Declan
  last_name: Schroeder
- first_name: Pavel
  full_name: Plevka, Pavel
  last_name: Plevka
citation:
  ama: 'Homola M, Büttner C, Füzik T, et al. Data: Structure and replication cycle
    of a virus infecting climate-modulating alga Emiliania huxleyi. 2024. doi:<a href="https://doi.org/10.5061/dryad.p5hqbzkw0">10.5061/dryad.p5hqbzkw0</a>'
  apa: 'Homola, M., Büttner, C., Füzik, T., Krepelka, P., Holbová, R., Chaillet, M.,
    … Plevka, P. (2024). Data: Structure and replication cycle of a virus infecting
    climate-modulating alga Emiliania huxleyi. Dryad. <a href="https://doi.org/10.5061/dryad.p5hqbzkw0">https://doi.org/10.5061/dryad.p5hqbzkw0</a>'
  chicago: 'Homola, Miroslav, Carina Büttner, Tibor Füzik, Pavel Krepelka, Radka Holbová,
    Marten Chaillet, Jiří Nováček, et al. “Data: Structure and Replication Cycle of
    a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” Dryad, 2024. <a
    href="https://doi.org/10.5061/dryad.p5hqbzkw0">https://doi.org/10.5061/dryad.p5hqbzkw0</a>.'
  ieee: 'M. Homola <i>et al.</i>, “Data: Structure and replication cycle of a virus
    infecting climate-modulating alga Emiliania huxleyi.” Dryad, 2024.'
  ista: 'Homola M, Büttner C, Füzik T, Krepelka P, Holbová R, Chaillet M, Nováček
    J, Förster F, Wilson W, Schroeder D, Plevka P. 2024. Data: Structure and replication
    cycle of a virus infecting climate-modulating alga Emiliania huxleyi, Dryad, <a
    href="https://doi.org/10.5061/dryad.p5hqbzkw0">10.5061/dryad.p5hqbzkw0</a>.'
  mla: 'Homola, Miroslav, et al. <i>Data: Structure and Replication Cycle of a Virus
    Infecting Climate-Modulating Alga Emiliania Huxleyi</i>. Dryad, 2024, doi:<a href="https://doi.org/10.5061/dryad.p5hqbzkw0">10.5061/dryad.p5hqbzkw0</a>.'
  short: M. Homola, C. Büttner, T. Füzik, P. Krepelka, R. Holbová, M. Chaillet, J.
    Nováček, F. Förster, W. Wilson, D. Schroeder, P. Plevka, (2024).
date_created: 2026-10-01T11:13:02Z
date_published: 2024-02-07T00:00:00Z
date_updated: 2026-10-01T11:15:29Z
day: '07'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.5061/dryad.p5hqbzkw0
fulldoi: https://doi.org/10.5061/dryad.p5hqbzkw0
main_file_link:
- open_access: '1'
  url: https://doi.org/10.5061/dryad.p5hqbzkw0
month: '02'
oa: 1
oa_version: None
publisher: Dryad
related_material:
  record:
  - id: '18920'
    relation: used_in_publication
    status: public
status: public
title: 'Data: Structure and replication cycle of a virus infecting climate-modulating
  alga Emiliania huxleyi'
type: research_data_reference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...