---
res:
  bibo_abstract:
  - The division of a mother cell into two daughter cells is a fundamental process
    in biology, involving the transfer of genomic information to the next generation
    of cells. In plant roots, cell divisions occur in the root apical meristem, a
    specialized tissue located at the plant’s root tip. Cell proliferation rates in
    the root apical meristem shape root growth, thereby contributing to general development
    as well as the acclimatization to stressful environmental conditions through phenotypic
    plasticity. Here, we present a protocol for the microscopical analysis of cell
    proliferation in Arabidopsis thaliana root meristems by 5-ethynyl-2ʹ-deoxyuridine
    (EdU) staining. EdU, a thymine-analog, is incorporated into newly synthesized
    DNA of proliferative cells during the S-phase of the cell cycle. Subsequently,
    EdU-containing nuclei can be labeled with a fluorophore and detected by confocal
    laser scanning microscopy. The combination of the EdU assay with cell wall staining
    enables the quantification of root meristem architecture parameters and cell division
    activity from microscopic images. Together, this can elucidate how root meristems
    respond to changes in environmental conditions or address fundamental questions
    of developmental plant biology.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Tilman
      foaf_name: Jacob, Tilman
      foaf_surname: Jacob
  - foaf_Person:
      foaf_givenName: Haiyue
      foaf_name: Ai, Haiyue
      foaf_surname: Ai
      foaf_workInfoHomepage: http://www.librecat.org/personId=71a6871f-c08f-11ee-886c-c975579c368c
  - foaf_Person:
      foaf_givenName: Marcel
      foaf_name: Quint, Marcel
      foaf_surname: Quint
  bibo_doi: 10.1007/978-1-0716-5603-7_13
  bibo_volume: 3086
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1940-6029
  dct_language: eng
  dct_publisher: Springer@
  dct_subject:
  - Root growth
  - Root development
  - Root meristem
  - Cell cycle
  - Cell division
  - S-phase
  - EdU
  - Arabidopsis thaliana
  - Microscopy
  dct_title: EdU-Based Quantitative Analysis of Cell Proliferation in Arabidopsis
    Root Meristems@
  fabio_hasPubmedId: '42814359'
...