---
res:
  bibo_abstract:
  - Plant grafting is a powerful tool in research and agriculture, enabling the study
    of long-distance signaling and improving crop performance. Hypocotyl grafting
    in Arabidopsis thaliana has become a key method for investigating inter-tissue
    communication, root-to-shoot transport, and vascular regeneration. Here, we describe
    a high-throughput hypocotyl grafting protocol that consistently achieves success
    rates above 90% while minimizing complications, such as adventitious root formation,
    through an optimized hypocotyl cutting position. Our streamlined approach eliminates
    the need for silicone tubes and fine forceps, requiring only a standard scalpel
    for the cutting and joining procedure. With proper training, an experienced user
    can graft up to 1000 plants per day, facilitating large-scale experiments. Additionally,
    we present vascular connectivity assays using fluorescent dyes and the adjuvant
    Adigor to enable phloem and xylem transport analysis across the graft junction
    without the need for wounding. By simplifying the grafting process while maintaining
    high efficiency, our protocol makes Arabidopsis grafting more accessible and scalable,
    facilitating research on long-distance molecular transport, vascular development,
    and plant biotechnology applications.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Kai
      foaf_name: Bartusch, Kai
      foaf_surname: Bartusch
      foaf_workInfoHomepage: http://www.librecat.org/personId=0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
    orcid: 0000-0003-2708-5545
  - foaf_Person:
      foaf_givenName: Elisabeth
      foaf_name: Truernit, Elisabeth
      foaf_surname: Truernit
  bibo_doi: 10.1007/978-1-0716-5488-0_33
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1064-3745
  - http://id.crossref.org/issn/1940-6029
  - http://id.crossref.org/issn/9781071654873
  dct_language: eng
  dct_publisher: Springer@
  dct_title: High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment
    in Arabidopsis Seedlings@
  fabio_hasPubmedId: '42681141'
...