---
OA_type: closed access
_id: '23126'
abstract:
- lang: eng
  text: Plant grafting is a powerful tool in research and agriculture, enabling the
    study of long-distance signaling and improving crop performance. Hypocotyl grafting
    in Arabidopsis thaliana has become a key method for investigating inter-tissue
    communication, root-to-shoot transport, and vascular regeneration. Here, we describe
    a high-throughput hypocotyl grafting protocol that consistently achieves success
    rates above 90% while minimizing complications, such as adventitious root formation,
    through an optimized hypocotyl cutting position. Our streamlined approach eliminates
    the need for silicone tubes and fine forceps, requiring only a standard scalpel
    for the cutting and joining procedure. With proper training, an experienced user
    can graft up to 1000 plants per day, facilitating large-scale experiments. Additionally,
    we present vascular connectivity assays using fluorescent dyes and the adjuvant
    Adigor to enable phloem and xylem transport analysis across the graft junction
    without the need for wounding. By simplifying the grafting process while maintaining
    high efficiency, our protocol makes Arabidopsis grafting more accessible and scalable,
    facilitating research on long-distance molecular transport, vascular development,
    and plant biotechnology applications.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Kai
  full_name: Bartusch, Kai
  id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
  last_name: Bartusch
  orcid: 0000-0003-2708-5545
- first_name: Elisabeth
  full_name: Truernit, Elisabeth
  last_name: Truernit
citation:
  ama: 'Bartusch K, Truernit E. High-Throughput Hypocotyl Grafting and Vascular Reconnection
    Assessment in Arabidopsis Seedlings. In: Liesche J, Sevanto S, eds. <i>Phloem</i>.
    2nd ed. MIMB. New York, NY: Springer; 2026:431-448. doi:<a href="https://doi.org/10.1007/978-1-0716-5488-0_33">10.1007/978-1-0716-5488-0_33</a>'
  apa: 'Bartusch, K., &#38; Truernit, E. (2026). High-Throughput Hypocotyl Grafting
    and Vascular Reconnection Assessment in Arabidopsis Seedlings. In J. Liesche &#38;
    S. Sevanto (Eds.), <i>Phloem</i> (2nd ed., pp. 431–448). New York, NY: Springer.
    <a href="https://doi.org/10.1007/978-1-0716-5488-0_33">https://doi.org/10.1007/978-1-0716-5488-0_33</a>'
  chicago: 'Bartusch, Kai, and Elisabeth Truernit. “High-Throughput Hypocotyl Grafting
    and Vascular Reconnection Assessment in Arabidopsis Seedlings.” In <i>Phloem</i>,
    edited by Johannes Liesche and Sanna Sevanto, 2nd ed., 431–48. MIMB. New York,
    NY: Springer, 2026. <a href="https://doi.org/10.1007/978-1-0716-5488-0_33">https://doi.org/10.1007/978-1-0716-5488-0_33</a>.'
  ieee: 'K. Bartusch and E. Truernit, “High-Throughput Hypocotyl Grafting and Vascular
    Reconnection Assessment in Arabidopsis Seedlings,” in <i>Phloem</i>, 2nd ed.,
    J. Liesche and S. Sevanto, Eds. New York, NY: Springer, 2026, pp. 431–448.'
  ista: 'Bartusch K, Truernit E. 2026.High-Throughput Hypocotyl Grafting and Vascular
    Reconnection Assessment in Arabidopsis Seedlings. In: Phloem. Methods in Molecular
    Biology, , 431–448.'
  mla: Bartusch, Kai, and Elisabeth Truernit. “High-Throughput Hypocotyl Grafting
    and Vascular Reconnection Assessment in Arabidopsis Seedlings.” <i>Phloem</i>,
    edited by Johannes Liesche and Sanna Sevanto, 2nd ed., Springer, 2026, pp. 431–48,
    doi:<a href="https://doi.org/10.1007/978-1-0716-5488-0_33">10.1007/978-1-0716-5488-0_33</a>.
  short: K. Bartusch, E. Truernit, in:, J. Liesche, S. Sevanto (Eds.), Phloem, 2nd
    ed., Springer, New York, NY, 2026, pp. 431–448.
date_created: 2026-10-13T11:14:23Z
date_published: 2026-09-03T00:00:00Z
date_updated: 2026-10-14T08:58:03Z
day: '03'
doi: 10.1007/978-1-0716-5488-0_33
edition: '2'
editor:
- first_name: Johannes
  full_name: Liesche, Johannes
  last_name: Liesche
- first_name: Sanna
  full_name: Sevanto, Sanna
  last_name: Sevanto
extern: '1'
external_id:
  pmid:
  - '42681141'
fulldoi: https://doi.org/10.1007/978-1-0716-5488-0_33
language:
- iso: eng
month: '09'
oa_version: None
page: 431-448
place: New York, NY
pmid: 1
publication: Phloem
publication_identifier:
  eisbn:
  - '9781071654880'
  eissn:
  - 1940-6029
  isbn:
  - '9781071654873'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in
  Arabidopsis Seedlings
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...