---
res:
  bibo_abstract:
  - Fluorescent dyes are often used to observe transport mechanisms in plant vascular
    tissues. However, it has been technically challenging to apply fluorescent dyes
    on roots to monitor xylem transport in vivo. Here, we present a fast, noninvasive,
    and high-throughput protocol to monitor xylem transport in seedlings. Using the
    fluorescent dyes 5(6)-carboxyfluorescein diacetate (CFDA) and Rhodamine WT, we
    were able to observe xylem transport on a cellular level in Arabidopsis thaliana
    roots. We describe how to apply these dyes on primary roots of young seedlings,
    how to monitor root-to-shoot xylem transport, and how to measure xylem transport
    velocity in roots. Moreover, we show that our protocol can also be applied to
    lateral roots and grafted seedlings to assess xylem (re)connection. Altogether,
    these techniques are useful for investigating xylem functionality in diverse experimental
    setups.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Kai
      foaf_name: Bartusch, Kai
      foaf_surname: Bartusch
      foaf_workInfoHomepage: http://www.librecat.org/personId=0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
    orcid: 0000-0003-2708-5545
  - foaf_Person:
      foaf_givenName: Noel
      foaf_name: Blanco-Touriñán, Noel
      foaf_surname: Blanco-Touriñán
  - foaf_Person:
      foaf_givenName: Antia
      foaf_name: Rodriguez-Villalón, Antia
      foaf_surname: Rodriguez-Villalón
  - foaf_Person:
      foaf_givenName: Elisabeth
      foaf_name: Truernit, Elisabeth
      foaf_surname: Truernit
  bibo_doi: 10.1007/978-1-0716-3477-6_1
  bibo_volume: 2722
  dct_date: 2023^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1064-3745
  - http://id.crossref.org/issn/1940-6029
  - http://id.crossref.org/issn/9781071634769
  dct_language: eng
  dct_publisher: Springer@
  dct_title: Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent
    Dyes@
  fabio_hasPubmedId: '37897596'
...