---
OA_type: closed access
_id: '23130'
abstract:
- lang: eng
  text: Fluorescent dyes are often used to observe transport mechanisms in plant vascular
    tissues. However, it has been technically challenging to apply fluorescent dyes
    on roots to monitor xylem transport in vivo. Here, we present a fast, noninvasive,
    and high-throughput protocol to monitor xylem transport in seedlings. Using the
    fluorescent dyes 5(6)-carboxyfluorescein diacetate (CFDA) and Rhodamine WT, we
    were able to observe xylem transport on a cellular level in Arabidopsis thaliana
    roots. We describe how to apply these dyes on primary roots of young seedlings,
    how to monitor root-to-shoot xylem transport, and how to measure xylem transport
    velocity in roots. Moreover, we show that our protocol can also be applied to
    lateral roots and grafted seedlings to assess xylem (re)connection. Altogether,
    these techniques are useful for investigating xylem functionality in diverse experimental
    setups.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Kai
  full_name: Bartusch, Kai
  id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
  last_name: Bartusch
  orcid: 0000-0003-2708-5545
- first_name: Noel
  full_name: Blanco-Touriñán, Noel
  last_name: Blanco-Touriñán
- first_name: Antia
  full_name: Rodriguez-Villalón, Antia
  last_name: Rodriguez-Villalón
- first_name: Elisabeth
  full_name: Truernit, Elisabeth
  last_name: Truernit
citation:
  ama: 'Bartusch K, Blanco-Touriñán N, Rodriguez-Villalón A, Truernit E. Monitoring
    Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes. In:
    <i>Xylem</i>. Vol 2722. 2nd ed. MIMB. New York, NY: Springer; 2023:3-15. doi:<a
    href="https://doi.org/10.1007/978-1-0716-3477-6_1">10.1007/978-1-0716-3477-6_1</a>'
  apa: 'Bartusch, K., Blanco-Touriñán, N., Rodriguez-Villalón, A., &#38; Truernit,
    E. (2023). Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using
    Fluorescent Dyes. In <i>Xylem</i> (2nd ed., Vol. 2722, pp. 3–15). New York, NY:
    Springer. <a href="https://doi.org/10.1007/978-1-0716-3477-6_1">https://doi.org/10.1007/978-1-0716-3477-6_1</a>'
  chicago: 'Bartusch, Kai, Noel Blanco-Touriñán, Antia Rodriguez-Villalón, and Elisabeth
    Truernit. “Monitoring Xylem Transport in Arabidopsis Thaliana Seedlings Using
    Fluorescent Dyes.” In <i>Xylem</i>, 2nd ed., 2722:3–15. MIMB. New York, NY: Springer,
    2023. <a href="https://doi.org/10.1007/978-1-0716-3477-6_1">https://doi.org/10.1007/978-1-0716-3477-6_1</a>.'
  ieee: 'K. Bartusch, N. Blanco-Touriñán, A. Rodriguez-Villalón, and E. Truernit,
    “Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent
    Dyes,” in <i>Xylem</i>, 2nd ed., vol. 2722, New York, NY: Springer, 2023, pp.
    3–15.'
  ista: 'Bartusch K, Blanco-Touriñán N, Rodriguez-Villalón A, Truernit E. 2023.Monitoring
    Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes. In:
    Xylem. Methods in Molecular Biology, vol. 2722, 3–15.'
  mla: Bartusch, Kai, et al. “Monitoring Xylem Transport in Arabidopsis Thaliana Seedlings
    Using Fluorescent Dyes.” <i>Xylem</i>, 2nd ed., vol. 2722, Springer, 2023, pp.
    3–15, doi:<a href="https://doi.org/10.1007/978-1-0716-3477-6_1">10.1007/978-1-0716-3477-6_1</a>.
  short: K. Bartusch, N. Blanco-Touriñán, A. Rodriguez-Villalón, E. Truernit, in:,
    Xylem, 2nd ed., Springer, New York, NY, 2023, pp. 3–15.
date_created: 2026-10-13T11:15:03Z
date_published: 2023-10-29T00:00:00Z
date_updated: 2026-10-14T10:33:41Z
day: '29'
doi: 10.1007/978-1-0716-3477-6_1
edition: '2'
extern: '1'
external_id:
  pmid:
  - '37897596'
fulldoi: https://doi.org/10.1007/978-1-0716-3477-6_1
intvolume: '      2722'
language:
- iso: eng
month: '10'
oa_version: None
page: 3-15
place: New York, NY
pmid: 1
publication: Xylem
publication_identifier:
  eisbn:
  - '9781071634776'
  eissn:
  - 1940-6029
  isbn:
  - '9781071634769'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent
  Dyes
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2722
year: '2023'
...